自体髂骨与胶原-羟基磷灰石复合重组人骨形成蛋白2修复大鼠单侧腭裂  

Autogenous iliac bone versus collagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite material for repair of unilateral cleft palate in rats

作　　者：沈悦 马海英 张彦升 王娟 时炳正 

机构地区：[1]沧州市人民医院口腔分院,河北省沧州市061001

出　　处：《中国组织工程研究》2015年第3期410-415,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省科技支撑计划项目(1213078ZD):单侧完全性唇腭裂患者上颌骨矫正后稳定性分析及治疗程序研究~~

摘　　要：背景:已有研究证实胶原-羟基磷灰石复合重组人骨形成蛋白2植骨效果较好,然而尚未有文献报道此复合材料与自体骨移植效果的对比评价。目的:检测自体髂骨移植与胶原-羟基磷灰石复合重组人骨形成蛋白2植骨在单侧完全性腭裂大鼠模型中的愈合效果。方法:首先建立32只SD大鼠单侧完全性腭裂模型,随机均分为两组,对照组于裂隙处移植自体髂骨,实验组移植胶原-羟基磷灰石复合重组人骨形成蛋白2生物材料,于移植后的第1,2,3,4周,检测血清中碱性磷酸酶和抗酒石酸酸性磷酸酶活性、新生上腭骨骨密度、成骨细胞特异性标志物骨钙素、骨保护素、核心结合因子及破骨细胞特异性标志物破骨细胞分化因子等基因的表达。结果与结论:随时间的推移,碱性磷酸酶活性逐渐升高,抗酒石酸酸性磷酸酶活性逐渐降低,实验组碱性磷酸酶活性始终高于对照组(P<0.05,P<0.01),抗酒石酸酸性磷酸酶活性始终低于对照组(P<0.05,P<0.01);骨密度逐渐增高,实验组始终高于对照组(P<0.01);骨钙素、骨保护素、核心结合因子基因水平逐渐上升,实验组始终高于对照组;破骨细胞分化因子逐渐降低,实验组始终低于对照组。表明胶原-羟基磷灰石复合重组人骨形成蛋白2复合生物材料植骨方式较自体植骨方式更有优势。BACKGROUND：It has been demonstrated that the effect of colagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft is good, but there is no comparative analysis of this composite graft and autologous bone graft. OBJECTIVE：To detect the healing effect of autologous bone graft versus colagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft in a rat model of unilateral complete cleft palate. METHODS： Firstly, we established the artificial unilateral complete cleft palate models in 32 Sprague-Dawley rats, and then the established animal models were randomly divided into control group and experimental group. The autologous iliac bone was transplanted into the fissures of control group, and the experimental group received colagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft. After that, the activities of serum alkaline phosphatase and tartrate-resistant acid phosphatase, bone mineral densities in the neonatal palate, expressions of osteocalcin, osteoprotegerin, core binding factor, and osteoclast differentiation＆amp;nbsp;factor were detected at 1, 2, 3, 4 weeks after treatment. RESULTS AND CONCLUSION： Over time, the alkaline phosphatase activity increased gradualy, while tartrate-resistant acid phosphatase activity decreased. Compared with the control group, the alkaline phosphatase activity was always higher （P 〈 0.05,P 〈 0.01） but the tartrate-resistant acid phosphatase activity was lower in the experimental group （P 〈 0.05,P 〈 0.01）; the bone mineral density increased in both groups, but it was always higher in the experimental group than the control group （P 〈 0.01）. The expression levels of osteocalcin, osteoprotegerin, and core-binding factor gene gradualy rose in both groups, but they were always higher in the experimental group than the control group; in contrast, the expression of osteoclast differentiation factor was decreased in both groups, and it was lower in the experimental group tha

关 键 词：生物材料 骨生物材料 单侧完全性腭裂 自体髂骨植骨 复合生物材料植骨 羟基磷灰石 重组人骨形成蛋白2 

分 类 号：R318[医药卫生—生物医学工程]