卡介菌多糖核酸干预豚鼠急性过敏反应的研究  

Intervention of BCG Polysaccharide and Nucleic Acid in Hypersusceptibility in Guinea Pigs

作　　者：唐何 李瑞莲 李子珊 陈子渊 欧阳杰湖 王蓉蓉 

机构地区：[1]湖南省食品药品检验研究院,长沙410001

出　　处：《中国药师》2015年第3期394-397,共4页China Pharmacist

基　　金：湖南省科技厅项目(编号:2011SK2018);湖南省自然科学基金项目(编号:11JJ4079)

摘　　要：目的:研究卡介菌多糖核酸对豚鼠急性过敏反应的作用及可能的机制,探讨卡介菌多糖核酸的质量控制效力模型的提高方法。方法:建立牛血清白蛋白诱导豚鼠产生急性过敏反应模型。激发后30 min内观察豚鼠全身反应,并根据致敏性症状评分标准分级。取血用ELISA法测致敏前、攻击前及攻击后豚鼠血清总Ig E和组胺水平。结果:攻击后,模型组和卡介菌多糖核酸低剂量组豚鼠出现不同程度的急性过敏反应症状,而卡介菌中、高剂量组豚鼠急性过敏反应症状明显缓解。模型组在攻击前、后采集的血清总Ig E水平分别为1.637 0±0.158 6,1.683 1±0.228 1μg·ml-1,较正常对照组显著升高(P<0.01),而同时给予卡介菌多糖核酸后,卡介菌高剂量和中剂量处理组在攻击前、后采集的血清总Ig E水平与模型组比较均显著降低(P<0.01)。经牛血清蛋白致敏后,在攻击前后,模型组血清中组胺水平分别为1.499 7±0.133 1,1.512 1±0.050 6μg·ml-1,与阴性对照组相比均显著升高(P<0.01),而同时给予卡介菌多糖核酸后,卡介菌高、中、低各剂量处理组在攻击前、后采集的血清中组胺水平与模型组比较有明显降低(P<0.01)。结论:卡介菌多糖核酸可剂量依赖性抑制牛血清蛋白引起的急性过敏反应。其抗急性过敏反应的可能机制与降低血清总Ig E和组胺水平有关。Objective：To study the effect and underlying mechanism of BCG polysaccharide and nucleic acid （ BCG-PSN） in hy-persusceptibility in guinea pigs to explore the improvement method for the quality control model of BCG-PSN. Methods：The ovalbumin induced hypersusceptibility animal model was established, the effect of BCG-PSN on hypersusceptibility in guinea pigs was observed. According to the guideline for immunity toxicity study on Chinese traditional medicine and natural medicine, the hypersusceptibility tests were carried out. Serum IgE and histamine were determined by ELISA. Results：The guinea pigs in the model group and the low dosage BCG-PSN group showed strong anaphylactic symptoms, while the middle and high dosage BCG-PNS groups showed fewer symp-toms. The level of IgE in the model group was （1. 673 0 ＆#177; 0. 158 6） μg＆#183;ml-1 and （1. 683 1 ＆#177; 0. 228 1）μg＆#183;ml-1 before and after the attacking, respectively, which was higher than that in the control group（P〈0. 01）. The levels of IgE in the middle and high dos-age BCG-PNS groups were decreased compared with those in the model group before and after the attacking（P〈0. 01）. The same re-sults were observed in the levels of histamine. Before and after the attacking, the levels of histamine in the model group was （1. 499 7 ＆#177; 0. 133 1） ng＆#183;ml-1 and （1. 512 1 ＆#177; 0. 050 6） ng＆#183;ml-1 , respectively, while the levels of histamine in low, middle and high dos-age BCG-PNS groups were decreased compared with those in the model group before and after the attacking（P〈0. 01）. Conclusion：BCG-PSN can dose-dependently inhibit the anaphylactic reaction induced by ovalbumin.

关 键 词：卡介菌多糖核酸 急性过敏反应 免疫球蛋白E 组胺 豚鼠 

分 类 号：R965.1[医药卫生—药理学]