干姜HPLC指纹图谱研究  

Study on the HPLC Fingerprint of Dry Ginger

作　　者：郭琪[1] 赵晓红 韩海建[3] 申睿[1] 杨莹[1] 雷虹[1] 李永申[1] 

机构地区：[1]北京军区联勤部药品仪器检验所,北京100071 [2]北京市通州区新华医院药剂科 [3]北京军区联勤部卫生部药材处

出　　处：《中国药师》2015年第3期397-399,共3页China Pharmacist

基　　金：军队中医药科研专项课题(编号:10ZYZ204)

摘　　要：目的:通过建立干姜HPLC指纹图谱,为干姜全面质量评价提供参考依据。方法:色谱柱为Agilent TC-C18(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1.0 ml·min-1,柱温为30℃,检测波长为240 nm,采用"中药指纹图谱相似度计算软件"进行处理。结果:10批干姜药材在240 nm下的色谱指纹图谱有10个共有峰,相似度在99%以上。结论:240 nm下干姜指纹图谱专属性及特征性较强,样品信息丰富,可作为干姜质量评价提供参考。Objective：To establish the HPLC fingerprint of dry ginger to provide experimental evidence for the quality control. Methods：An Agilent TC-C18 column （250 mm ＆#215; 4. 6 mm,5 μm） was used, the flow phase was acetonitrile-water with gradient elu-tion, the flow rate was 1. 0 ml＆#183;min-1 , the column temperature was 30℃, and the detection wavelength was 240 nm. The data were e-valuated by the similarity evaluation software for TCM fingerprint. Results：There were 10 common peaks in HPLC chromatogram of 10 batches of dry ginger at 240 nm, and the chemical similar coefficient was 99%. Conclusion： The fingerprint of ginger at 240 nm is highly specific and typical with a rich fund of information, which can provide useful references for the quality control and evaluation of dry ginger.

关 键 词：干姜 指纹图谱 高效液相色谱法 

分 类 号：TQ460.72[医药卫生—药物分析学]