白背叶根质量标准的建立  

Establishment of Quality Standard for Malloti Apeltae Radix

作　　者：唐秀玲 罗轶 黄清泉 唐秀利 

机构地区：[1]广西食品药品检验所,南宁530021 [2]柳州市柳铁中心医院

出　　处：《中国药师》2015年第3期508-511,共4页China Pharmacist

摘　　要：目的:完善和提高白背叶根的质量标准。方法:显微鉴别白背叶根横切面和粉末;采用TLC法对白背叶根水溶性成分和脂溶性成分进行定性鉴别;特征图谱采用HPLC法以芦丁为参照物确定各特征峰的相对保留时间及相对峰面积,色谱柱:SPOLAR C18柱(250 mm×4.6 mm,5μm),流动相:乙腈-0.4%磷酸溶液梯度洗脱;检测波长为328 nm;流速:1.0 ml·min-1;柱温:35℃。结果:显微特征明显;薄层色谱斑点圆整、清晰、重复性好。特征图谱供试品色谱呈现5个特征峰,与参照物(芦丁)峰的相对保留时间应在规定值±5%之内,其中峰3、峰4相对峰面积不低于0.23和0.66。结论:所建立的方法简便快捷,重复性好,结果准确可靠,能有效控制白背叶根质量,可作为白背叶根的质量标准的主要控制指标。Objective：To perfect and improve the quality standard of Malloti Apeltae Radix. Methods：Microscopic identification was used to identify the transverse section and the powder. The water-soluble and the fat-soluble components were identified by TLC. Rutin was used as the reference in the characteristic chromatogram established by HPLC to determine the relative retention time and rel-ative peak area of each characteristic absorption band. A SPOLAR C18 chromatographic column （ 250 mm ＆#215; 4. 6 mm,5μm） was used with acetonitrile-0. 4% phosphate solution as the mobile phase with gradient elution, the detection wavelength was set at 328 nm, the flow rate was 1. 0 ml＆#183;min-1, and the temperature was 35℃. Results： The microscopic characteristics were obvious. The spots of TLC were round and clear with good repeatability. Five characteristic absorption bands were shown in the fingerprints with the relative retention time within ＆#177; 5% of reference value of rutin, and the relative area of peak 3 and peak 4 was not lower than 0. 23 and 0. 66, respectively. Conclusion：The method is convenient, fast and repeatable, and the result is accurate and reliable, which can be used to control the quality of Malloti Apeltae Radix effectively, and as the main index of the quality standard.

关 键 词：白背叶根 显微鉴别 薄层色谱法 高效液相色谱法 特征图谱 

分 类 号：R282.5[医药卫生—中药学]