可降解聚合物抗菌纤维膜的原位制备及性能表征  

In Situ-preparation and Properties of Doped Zn O Biodegradable Polymer Fibers

作　　者：单晓茜[1] 刘昌胜[2] 欧阳春发[1] 高群[1] 施宇涛[1] 郑康生[1] 

机构地区：[1]上海应用技术学院材料科学与工程学院,上海201418 [2]华东理工大学材料科学与工程学院教育部医用生物材料工程研究中心,上海200237

出　　处：《科学技术与工程》2015年第4期180-185,共6页Science Technology and Engineering

基　　金：上海市自然科学基金(11ZR1435800);国家自然科学基金(31100673)资助

摘　　要：以二氯甲烷、N,N-二甲基甲酰胺为溶剂,以聚乳酸(PLA)为纺丝纤维基体,添加纳米氧化锌(Zn O)和表面活性剂吐温60,利用高压静电纺丝法,原位制备纳米Zn O/PLA复合纤维膜。所得纤维膜中,Zn O分散均匀,纤维平均直径约300 nm,且表面光滑。纺丝纤维的亲水性随着纳米Zn O的增加而大幅提高,断裂强度和延伸率随纳米Zn O含量的增加呈现先上升后下降的趋势。抗菌能力也呈现一定的Zn O含量依赖性。细胞毒性和细胞黏附实验表明,该抗菌纤维膜具有较好的细胞相容性。The nano-zinc oxide（ Zn O,50nm） doped polylactic acid（ PLA） nanofibers were fabricated via insitu electrospinning technique. The mixture of dichloromethane（ DCM） and n,n-dimethyl formamide（ DMF） were pre-prepared for the solvent of PLA,then the nano-zinc oxide particles have been mixed into the solvents mixture.After optimazing the preparation procedure,the Zn O particles were well-distributed in the PLA solvents through observed by TEM. The structure of Zn O / PLA composite nanofibers has been observed by SEM,and the diameter was about 300 nm. Meanwhile,the so spun fibers were smooth and uniform without any bead-like structure The mechanical properties of composite nanofibers have been demonstrated by a tensile test,and the results demonstrated that the breaking strength and elongation rate were increased with the percentage of nano-Zn O. It showed that the addition of nano-Zn O may be an effective way to toughen the PLA nanofibers. Additionally,the hydrophilicity of the compound nanofibers has been recorded through a contact angle apparatus. It showed that the hydrophilicity of compound nanofibers were decreased monotonically with the increasing nano-Zn O content,obviously. Then,the cytotoxicity and the antibacterial properties have also been examined. These results suggested that the Zn O / PLA nanofibers were safe to cells and SEM observation also showed that cells had spread well onto the fiber mats with a high level of cell / scaffold interaction; this was demonstrated by the numerous pseudopodia and cell secretions that emerged. Finally,the ability to antibacterial was investigated through a Bacteriostatic ring experiment which showed that the antibacterial property had a Zn O-concentration dependent manner.

关 键 词：聚乳酸 纳米氧化锌 静电纺丝 扫描电子显微镜 拉伸强度 抗菌性能 

分 类 号：O631[理学—高分子化学]