抵抗素基因多态性与2型糖尿病关联性分析  

Association between RETN gene polymorphisms and type 2 diabetes mellitus

作　　者：修良昌[1] 任寒 杨杰[1] 林美华[1] 欧阳平[1] 孔丹莉[1] 饶绍奇[1] 丁元林[1] 

机构地区：[1]广东医学院公共卫生学院流行病与卫生统计学教研室,广东东莞523808

出　　处：《中国公共卫生》2015年第3期369-372,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81273166);广东省自然科学基金(S2012010008271);东莞市科技计划项目(2012105102010);广东医学院科研基金(M2013042)

摘　　要：目的探讨广东省汉族人群中抵抗素基因(RETN)的单核苷酸多态性(SNP)与2型糖尿病的关联。方法采用以医院为基础病例对照研究方法对2010年11月—2012年7月在广东省8家医院内分泌科就诊的595例2型糖尿病患者和同期在此8家医院健康体检的725名体检人群进行问卷调查,并采用SNPscanTM多重SNP分型试剂盒对筛选的2个tag SNP(rs7408174、rs3219175)进行基因分型;应用χ2检验、logistic回归模型等方法分析SNP位点与2型糖尿病的关联性。结果 rs7408174的TT、CT、CC基因型频数在病例组为329例(55.3%)、230例(38.7%)、36例(6.1%),在对照组为366人(50.8%)、308人(42.8%)、46人(6.4%);rs3219175位点的GG、GA、AA基因型频数在病例组为377例(63.4%)、200例(33.6%)、18例(3.0%),在对照组中为442人(61.0%)、253人(34.9%)、30人(4.1%);调整性别、体质指数和甘油三酯等因素后,rs7408174、rs3219175在等位基因关联分析和基因型关联分析中差异均无统计学意义(P>0.05)。结论广东省汉族人群rs7408174和rs3219175与2型糖尿病无关联。Objective To evaluate the association between two single nucleotide polymorphisms（ SNPs） located in RETN gene and type 2 diabetes mellitus（ T2DM） in Han population in Guangdong province. Methods Two tag SNPs（ rs7408174 and rs3219175） were picked out from RETN gene. Then SNP genotyping was performed using SNPscanTMKit in 595 cases and 725 controls. Chi-square test,logistic regression model were employed in statistical analyses. Results The frequencies of TT,CT and CC of rs7408174 were 329（ 55. 3%）,230（ 38. 7%）,and 36（ 6. 1%） in the cases and 366（ 50. 8%）,308（ 42. 8%）,and 46（ 6. 4%） in the controls,respectively. The frequencies of GG,GA and AA of rs3219175 were 377（ 63. 4%）,200（ 33. 6%）,and 18（ 3. 0%） in the cases and 442（ 61. 0%）,253（ 34. 9%）,and 30（ 4. 1%） in the controls,respectively. No association between SNP of rs7408174 and T2 DMwas found in allelic association analysis and genotypic association analysis after adjustment for sex,body mass index and triglyceride. The other SNP of rs3219175 was not associated with T2 DMeither. Conclusion Neither rs7408174 nor rs3219175 of RETN gene was associated with T2 DM.

关 键 词：2型糖尿病 抵抗素 单核苷酸多态性 

分 类 号：R587.1[医药卫生—内分泌]