甘薯悬浮细胞系的建立及对抗草甘膦基因的遗传转化  

Establishment of Cell Suspension and Transformation of Herbicide Resistant EPSPS Gene

作　　者：张聪[1] 蒲志刚[1] 张婷[1] 李明[1] 屈会娟[1] 谭文芳[2] 杨松涛[2] 王大一[2] 阎文昭[1] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066 [2]四川省农业科学院作物研究所,四川成都610066

出　　处：《西南农业学报》2015年第1期41-44,共4页Southwest China Journal of Agricultural Sciences

基　　金：四川省财政创新能力提升工程青年基金项目(2013QNJJ-003);四川省农作物育种攻关项目甘薯育种子课题(2011-2015);四川省基因工程育种项目(2011JYGC01-005)

摘　　要：选取8个四川主栽甘薯品种为材料,剥离茎尖诱导胚性愈伤组织,建立胚性细胞悬浮培养系。以烟草叶片和川薯20悬浮细胞为受体材料,利用农杆菌浸染方法转化抗除草剂基因EPSPS。结果表明,经PCR检测及草甘膦喷施试验后,获得了抗草甘膦阳性甘薯植株13株、阳性烟草植株25株,此实验为以EPSPS基因作为遗传转化的标记基因提供了帮助。Eight Sichuan＇s main varieties of sweet potato were used as tested materials,and their embryonic callus induction and suspension cell culture system by stripping stem tip was established,then plant expression vectors containing herbicide resistance gene EPSPS were introduced into tobacco leaves and chuanshu20 suspension cell by agrobacterium-mediated transformation. The result showed that by PCR analysis and herbicide spraying test,13 positive sweet potato plants and 25 positive tobacco plants of herbicide resistance were observed,which provided a help for herbicide resistance EPSPS gene as a marker gene in transformation.

关 键 词：甘薯 悬浮细胞 草甘膦 除草剂 遗传转化 

分 类 号：S531[农业科学—作物学]