江南越桔组织培养和快繁技术研究  

Study on Tissue Culture and Rapid Propagation Technique of Vaccinium mandarinorum Hance Meristem

作　　者：杨春梅[1] 吴丽芳[1] 汪国鲜[1] 屈云慧[1] 单芹丽[1] 曹桦[1] 阮继伟[1] 李金泽[1] 蒋海玉[2] 

机构地区：[1]云南省农业科学院花卉研究所/云南省花卉育种重点实验室/国家观赏园艺工程技术研究中心,云南昆明650205 [2]云南省农业科学院农业经济信息研究所,云南昆明650205

出　　处：《西南农业学报》2015年第1期344-348,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家农业科技成果转化项目(2011GB2F300002);昆明市科技计划项目(11N010409)

摘　　要：利用江南越桔的茎段为外植体,进行其组培快繁技术体系研究。结果表明,WPS+6-BA 3.0 mg/L+NAA 0.2 mg/L+蔗糖20 g/L为诱导丛生芽较佳的启动培养基;WPS+ZT 1.0 mg/L+6-BA 0.2 mg/L+NAA 0.5 mg/L+蔗糖30 g/L为组培苗叶片诱导不定芽增殖较佳的培养基;1/2MS+IBA 0.2 mg/L+NAA 0.5 mg/L+活性炭3.5 mg/L为丛生芽生根较佳的培养基。The stem segments of Vaccinium mandarinorum were used as the explants to study the propagation via tissue culture. The results showed that the WPS ＋ 6-BA 3. 0 mg / L ＋ NAA 0. 2 mg / L ＋ sucrose 20 g / L was the optimum medium for inducing cespitose buds. WPS＋ ZT 1. 0 mg / L ＋ 6-BA 0. 2 mg / L ＋ NAA 0. 5 mg / L ＋ sucrose 30 g / L was the optimum medium for cespitose buds multiplication by the leaf of tissue culture seedlings. 1 /2MS ＋ IBA 0. 2 mg / L ＋ NAA 0. 5 mg / L ＋ activated carbon 3. 5 mg / L was the optimum medium for buds rooting.

关 键 词：江南越桔 不定芽 组织培养 正交实验 方差分析 

分 类 号：S663.9[农业科学—果树学]