脂肪酸转运蛋白4(SLC27A4)基因内含子8多态性研究  

Study on Polymorphisms of Intron 8 in Pig SLC27A4 Gene

作　　者：贾坤航[1] 邢晋祎[1] 邹士涛[1] 李洁[1] 

机构地区：[1]临沂大学生命科学学院,山东临沂276005

出　　处：《西南农业学报》2015年第1期386-389,共4页Southwest China Journal of Agricultural Sciences

基　　金：山东省自然科学基金(ZR2013CL012);临沂大学国家级大学生创新创业训练计划项目(201310452065)

摘　　要：脂肪酸转运蛋白4（SLC27A4）是主要的极长链脂肪酰基辅酶A合成酶,在脂质代谢中起着重要作用。本文利用PCR-RFLP方法分析了6个猪种的SLC27A4基因内含子8的多态性。结果表明,在长白猪群体中检测到AA、AG和GG 3种基因型;在莱芜猪和大约克检测到AG和GG 2种基因型;其余猪种只检测到GG 1种基因型。在6个猪群中G均为优势等位基因,GG为优势基因型。χ2检验显示,基因型分布在莱芜猪、大约克和长白猪群均达到了哈代—温伯格平衡（P〉0.05）;不同基因型在群体间的分布差异极显著（P〈0.01）。群体遗传特性结果表明,有效等位基因数在1.000~1.2914之间。多态信息含量（PIC）介于0.0000~0.2002之间,为低度多态。Solute carrier family 27（ fatty acid transporter）,member 4,is the principal very long chain fatty acyl-co A synthetase which plays a role in lipid metabolism. The distributions of Msp I polymorphisms of intron 8 in SLC27A4 were assayed by PCR-RFLP. The results showed that genotypes of SLC27A4 were AA,AG and GG in Landrace,AG and GG in Laiwu and Yorkshire,and only GG in Licha,Yimeng and Duroc pigs. Allele G and genotype GG were dominant in all 6 breeds. A chi-square analysis indicated that the genotype distribution in Laiwu,Yorkshire and Landrace pigs were in Hardy-Weinberg equilibrium（ P 0. 05）. Distributions of AA,AG and GG genotypes were significantly different in 6 breeds（ P 0. 01）. Population genetic character analysis indicated that effective number of alleles ranged from 1. 000 to 1. 2914,and polymorphism information content（ PIC） was between 0. 0000 and 0. 2002,being low polymorphic locus.

关 键 词：猪 SLC27A4基因 多态性 遗传多样性 PCR-RFLP 

分 类 号：S828[农业科学—畜牧学] Q341[农业科学—畜牧兽医]