大麻雄性基因连锁AFLP分子标记的筛选及鉴定  

Screening and Identification of AFLP Markers Linked to Male Gene in Cannabis sativa L.

作　　者：郭丽[1] 张海军[1,2,3] 王明泽[1] 车野[1] 刘德泉[3] 陈井生[1] 刘德福[1] 于吉东[1] 吴耀坤[1] 

机构地区：[1]黑龙江省农业科学院大庆分院,黑龙江大庆163316 [2]吉林省种子管理总站,长春130062 [3]吉林大学植物科学学院,长春130062

出　　处：《中国麻业科学》2015年第1期5-8,共4页Plant Fiber Sciences in China

基　　金：国家麻类产业技术体系项目(CARS-19-S04);黑龙江省农业科技创新工程重点(青年基金项目)(2012QN002)

摘　　要：为建立一种大麻早期性别筛选及鉴定方法,利用AFLP标记技术,筛选了64对Eco RI-NNN/Mse I-NNN引物组合,对11个不同大麻品种雌﹑雄植株的混合DNA池进行了性别连锁特异性条带的筛选。结果表明,6对引物组合表现出多态性,其中,Eco RI-ACA/Mse ICTG在雄性DNA池中扩增出1条特异条带,经各品种单株DNA验证,该条带只在雄性单株稳定出现,回收﹑克隆﹑测序后获得1条可用于大麻早期田间性别鉴定的734bp雄性特异条带。64 pairs of AFLP primer combinations of 8 pairs of Eco RI- NNN and Mse I- NNN primers were used to find the polymorphisms between mixed DNA pools of Cannabis sativa L. varieties. Six pairs of sixty- four primer combinations showed polymorphism between male and female DNA pools.700 bp specific fragments were amplified in the primer combination of Eco RI- ACA and Mse I- CTG in male DNA pool. This marker was testified with individual DNA of 11 varieties,and the fragment could only be amplified in male plants. The male- specific fragment of 734 bp was recovered,cloned and sequenced.

关 键 词：大麻 雄性 AFLP 分子标记 

分 类 号：S563.3[农业科学—作物学]