亚麻与黄麻沤麻池的细菌生物多样性分析  

Analysis On Bacteria Biodiversity in Flax and Jute Retting Pond

作　　者：张兴群[1,2] 原月梦 孔德枝 郁崇文[1,2,3] 金关荣[4] 傅佳佳[5] 丁若垚[1,2,3] 

机构地区：[1]东华大学纺织面料技术教育部重点实验室,上海201620 [2]东华大学生态纺织教育部重点实验室,上海201620 [3]东华大学纺织学院,上海201620 [4]浙江省农业科学院萧山棉麻研究所,杭州311202 [5]江南大学生态纺织教育部重点实验室,江苏无锡214122

出　　处：《中国麻业科学》2015年第1期14-20,共7页Plant Fiber Sciences in China

基　　金：中国博士后科学基金面上资助(2014M561386);国家麻类产业技术体系建设专项资金资助项目(CARS-19);国家自然科学基金项目(31201134);江苏省基础研究计划青年基金项目(BK2012112);东华大学中央高校基本科研业务费专项资金(14D110524);大学生"小平科技创新团队"(麻类纤维生物脱胶技术产业化集成研发团队)

摘　　要：本研究以亚麻与黄麻两用沤麻池中的沤麻液为研究对象,取样后富集培养分离得到9株脱胶细菌,通过传统微生物学研究手段与分子生物学系统发育分析相结合的方法进行生物多样性研究。研究结果表明:9种细菌分别聚类为产碱菌属(Alcaligenes sp.)4株,芽孢杆菌属(Bacillus sp.)4株,寡氧单胞菌属(Stenotrophomonas sp.)1株。系统发育分析发现9种细菌从亲缘关系上较为接近单胞菌属Stenotrophomonas sp.,假单胞菌属Pseudomonas sp.和芽孢杆菌属Bacillus sp.。这三类菌属也是目前已知细菌中较为常见的脱胶菌属。说明该9种细菌与脱胶菌属关系较近,具有进一步研究的可能。In this study,bacteria strains isolated from flax and jute retting pond were studied. Traditional microbiology method and molecular biology method were used to analyze bacteria biodiversity.The results showed that 9 isolated strains of bacteria belong to members of 3 genuses that four strains are classified into Alcaligenes sp.,four strains are classified into Bacillus sp. and one strain are classified into Stenotrophomonas sp.. Phylogenetic analysis showed that all these strains are close to Stenotrophomonas sp.,Pseudomonas sp. and Bacillus sp. which were recognized as degumming bacteria in genetic relationship.

关 键 词：亚麻 黄麻 沤麻 细菌 生物多样性 

分 类 号：S154.381[农业科学—土壤学]