RP-HPLC法测定橘红化痰丸中柚皮苷的含量  

Determination of naringin in Juhong Huatan pills by RP-HPLC

作　　者：司徒伟良 陈琳[1] 李海斌[1] 宋粉云[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2015年第1期43-45,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金资助项目(5002841)

摘　　要：目的建立测定橘红化痰丸中柚皮苷含量的方法。方法采用反相高效液相色谱法,色谱柱为Diamonsil-C18柱,流动相为甲醇-0.2%（体积分数）H3PO4水溶液,梯度洗脱,流速为1.0 mL·min^-1,检测波长为283 nm,柱温为30℃。结果柚皮苷质量浓度在5.2～52.0μg·mL^-1范围内具有良好的线性关系（r=0.999 7）,柚皮苷平均回收率为101.3%,RSD值为0.47%（n=6）。结论本方法可用于橘红化痰丸中柚皮苷的质量分数测定。Objective To establish the determination method for naringin in Juhong Huatan pills. Methods An RP-HPLC method was established. The chromatographic column was Diamonsil-C18. The mobile phase was a mixture of methanol and 0. 2% phosphoric acid with gradient elution at a flow rate of 1. 0 mL · min^-1. The detection wavelength was set at 283 nm,and the column temperature was maintained at 30 ℃. Results The linear range of naringin was 5. 2-52. 0 μg·m L^-1（ r= 0.999 7）. The average recovery was 101.3%,with RSD of0.47%（ n = 6）. Conclusion The method could be used for the quantitative determination of the preparation.

关 键 词：橘红化痰丸 柚皮苷 反相高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]