HPLC法测定人参首乌胶囊中大黄素、大黄素甲醚的含量  

Determination of emodin and physcion in Renshen Shouwu capsules by HPLC

作　　者：曾亮[1] 赵文静[1] 罗展远 贝伟剑[1] 

机构地区：[1]广东药学院中医药研究院,国家中医药管理局高脂血症调肝降脂重点研究室/脂代谢三级实验室,广东省代谢性疾病中医药防治重点实验室,广东广州510006

出　　处：《广东药学院学报》2015年第1期46-49,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省教育部产学研结合项目(2012B091100193);广州市科技计划助资项目(11C32130738)

摘　　要：目的建立高效液相色谱法测定人参首乌胶囊中大黄素及大黄素甲醚的质量分数。方法采用Diamonsil C18（250 mm×4.6 mm,5μm）色谱柱,以甲醇-0.1%（体积分数）H3PO4（体积比80∶20）为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为254 nm。结果大黄素、大黄素甲醚分别在0.03～1.20μg、0.04～1.60μg范围内与峰面积呈良好的线性关系,相关系数r均为0.999 9。大黄素和大黄素甲醚的平均回收率为100.25%（RSD为1.17%）和97.52%（RSD为2.52%）。结论本方法专属性强、重复性好,可用于人参首乌胶囊中大黄素和大黄素甲醚质量分数的测定。Objective To establish an HPLC method for the determination of emodin and physcion in Renshen Shouwu capsules. Methods The separation was performed on Diamonsil C18（ 250 mm×4.6 mm,5 μm） column eluted with a mobile phase of methanol-0.1% phosphoric acid solution（ 80 ∶ 20）. The flow rate was 1.0 mL / min and column temperature was 30 ℃. The detection wavelength was set at 254 nm.Results The calibration curve was linear within the range of 0.03-1.20 μg for emodin,and 0.04-1.60 μg for physcion,and both correlation coefficients were 0. 999 9. The average recoveries were 100. 25%（ RSD =1. 17%） for emodin,and 97.52%（ RSD = 2.52%） for physcion,respectively. Conclusion This method is accurate and reproducible for the determination of emodin and physcion in Renshen Shouwu capsules.

关 键 词：人参首乌胶囊 大黄素 大黄素甲醚 HPLC法 含量测定 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]