金黄色葡萄球菌生物膜形成规律及清开灵颗粒对其影响研究  

Formation rule of Staphylococcus aureus biofilm and effect of Qingkailing granules on this biofilm

作　　者：李将[1] 刘广锋[2] 李瑶[1] 员荣 邓婉婷[1] 管淑玉[1] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]中国水产科学研究院南海水产研究所,广东广州510300

出　　处：《广东药学院学报》2015年第1期114-116,131,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金项目(81001702);省部产学研结合项目(2010B090400519);广东省大学生创新训练计划项目(1057312033)

摘　　要：目的观察金黄色葡萄球菌生物膜形成规律以及清开灵颗粒对金黄色葡萄球菌生物膜的抑制作用和破坏作用。方法倍比稀释法测定清开灵颗粒最小抑菌浓度（MIC）,结晶紫染色法测定同时加入菌液和药液对生物膜的抑制作用,结晶紫染色法测定清开灵颗粒对金黄色葡萄球菌生物膜的破坏作用。结果15 h左右生物膜形成较好。生物膜抑制实验和破坏实验表明在3.9～62.4 mg·mL^-1浓度范围内清开灵颗粒具有良好的作用,且与阴性对照组差异有统计学意义（P〈0.05）。结论清开灵颗粒对金黄色葡萄球菌具有良好的抑菌效果,对其生物膜的形成具有抑制和破坏作用,且呈浓度依赖性。Objective To observe the formation rule of Staphylococcus（ S.） aureus biofilm and the inhibitory and damage effects of Qingkailing granules on this biofilm. Methods Double dilution method for the determination of minimum inhibitory concentration（ MIC） of Qingkailing granules. The inhibitory effect on biofilm was examined by crystal violet assay,while bacteria and sample liquid were joined in the biofilm at the same time. The damage effect on biofilm was also examined by crystal violet assay,while Qingkailing granules was added after S. aureus biofilm formation. Results The biofilms formed better in 15 hours.Qingkailing granules showed a good effect with 3. 9 to 62. 4 mg · mL^-1concentration proved by biofilm inhibition test and destruction test（ P〈0. 05）. Conclusion Qingkailing granules exhibits a good antibacterial effect on S. aureus,it also has a inhibitory and damage effect on S. aureus biofilm in a concentration-dependent manner.

关 键 词：清开灵颗粒 金黄色葡萄球菌 生物膜 

分 类 号：R285[医药卫生—中药学]