Militarine对离体大鼠胸主动脉环的舒张作用及机制研究  

Effect and Mechanism of Militarine in Relaxing Isolated Thoracic Aorta Rings in Rats

作　　者：徐晓雪[1] 徐广涛[1] 李小鹏[1] 钟旭[1] 韩伟健[1] 许枬[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中华中医药学刊》2015年第3期617-620,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家科技支撑计划项目(2012BAI28B00)

摘　　要：目的:研究从手掌参中分离的Militarine对离体大鼠胸主动脉环的舒张作用并探讨其可能的机制。方法:记录去甲肾上腺素(NE)和KCl预收缩的离体大鼠主动脉环张力变化,观察Militarine的舒血管作用及不同工具药对其作用的影响。结果:Militarine可以抑制去甲肾上腺素(NE,1×10-4mol·L-1)和KCl(1.8 mol·L-1)引起去内皮和内皮完整的离体大鼠胸主动脉环的收缩作用,但二者有明显差别;L-硝基精氨酸甲酯(LNAME,1×10-4mol·L-1)、亚甲蓝(MB,1×10-5mol·L-1)、吲哚美辛(INDO,1×10-5mol·L-1)均能一定程度抑制Militarine对胸主动脉环的舒张作用;钾离子通道阻断剂四乙基胺(TEA,1×10-3mol·L-1)、格列苯脲(glibenclamide,1×10-5mol·L-1)、Ba Cl2(1×10-4mol·L-1)可明显减弱Militarine对血管环的舒张作用;在无钙环境下,预孵育Militarine对去甲肾上腺素引起的血管环收缩有明显抑制作用。结论:Militarine有内皮依赖性和非内皮依赖性的血管舒张作用,其作用机制可能涉及激活内皮细胞中NO/c GMP通路,开放钾离子通道和抑制钙离子内流。Objective： To investigate the effect of Militarine in relaxing isolated thoracic aorta rings in rats and to discuss its possible mechanism. Method： Isotonic tension of isolated thoracic aortic rings in rats with norepineprine（ NE）and KCl was recorded to observe the vasodilatory effect of Militarine on it. Result： Militarine had the effect in relaxing thoracic aortas with or without endothelium and there was significant difference. NG- nitro- L- argininemethylester（ L-NAME,1 × 10- 4mol·L- 1）,methylene blue（ MB,1 × 10- 5mol·L- 1）,indomethacin（ INDO,1 × 10- 5mol·L- 1） had effect on the vasodilation action of Militarine on thoracic aortas precontracted by NE. Tetrathylamonium（ TEA,1 × 10- 3mol·L- 1）,Ba Cl2（ 1 × 10- 1mol·L- 1） and glibenclamide（ Gli,1 × 10- 5mol·L- 1） could inhibit Militarine＇s effect in relaxing thoracic aorta rings. In the absence of Ca2 ＋,pre-incubated milirarine showed a notable inhibitory effect in relaxing NE contraction. Conclusion： Militarine shows a dose-dependent and endothelium- dependent effect in relaxing rat isolated thoracic aorta rings. The mechanism is related to signal of NO / c GMP in endothelium and K＋channel,release inhibition of Ca2 ＋stored in endoplasmic reticulum of vascular smooth muscle cells and inflow of external calcium Ca2 ＋.

关 键 词：Militarine 手掌参 胸主动脉环 血管舒张 钾通道阻滞剂 钙通道 

分 类 号：R285.5[医药卫生—中药学]