中药对胰岛素抵抗多囊卵巢综合征大鼠卵巢组织胰岛素受体及其底物表达的初步研究  

Preliminary Study on the Effort of the Traditional Chinese Medicine(TCM) on the Expression of Insulin Receptor and its Substrate-1 in Ovarian Tissue of the Polycystic Ovary Syndrome Rats with Insulin Resistance

作　　者：王昕[1] 

机构地区：[1]辽宁中医药大学附属医院妇产科,辽宁沈阳110032

出　　处：《中华中医药学刊》2015年第3期630-632,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省科技厅基金项目(2013226012)

摘　　要：目的:观察抑囊助孕汤对胰岛素抵抗多囊卵巢综合征(PCOS+IR)大鼠卵巢组织内胰岛素受体(InsR)和胰岛素受体底物1(IRS-1)蛋白表达的影响,探讨其改善胰岛素抵抗的分子机制。方法:以丙酸睾酮皮下注射,配合高脂高卡饲料诱导SD雌性大鼠建立PCOS+IR大鼠模型,以抑囊助孕汤干预治疗20天,用免疫沉淀和western blot方法检测抑囊助孕汤治疗后PCOS+IR大鼠卵巢组织内InsR和IRS-1蛋白表达水平。结果:抑囊助孕汤治疗PCOS+IR大鼠后,卵巢组织内InsR和IRS-1表达较对照组增加。结论:抑囊助孕汤促进PCOS+IR大鼠卵巢组织InsR和IRS-1蛋白水平的表达,这可能是改善组织胰岛素敏感性的机制之一。Objective： To observe the effort of ＂Yi Nang Zhu Yun Tang＂on the protein expression of Insulin receptor（ InsR） and Insulin receptor substrate- 1（ IRS- 1） in the ovarian tissue of polycystic ovary syndrome rats with insulin resistance so as to explore the molecular mechanism of the improving of Insulin Resistance. Methods： With Testosterone Propionate subcutaneous injection,cooperating with high fat and high card feed to induce SD female rats to build the PCOS ＋ IR rat model. Using ＂Yi Nang Zhu Yun Tang＂for treatment in the later 20 days as the treatment group. And then Using Immune precipitation and western blot method to detect the protein expression level of in InsR and IRS- 1 in the ovarian tissues. Results： The protein expession level of InsR and IRS- 1 are higher in the treatment group,which may be one of the mechanism of improving tissue insulin sensitivity.

关 键 词：抑囊助孕汤 胰岛素受体 胰岛素受体底物-1 

分 类 号：R285.5[医药卫生—中药学]