电针经穴对脑缺血再灌注大鼠海马促肾上腺皮质激素释放因子和皮质醇mRNA表达影响  

Effects of electroacupuncture at meridian point on the mRNA expressions of corticotropinreleasing factor and corticosteroid in the hippocampus after cerebral ischemia reperfusion in rats

作　　者：任路明 蔡玉颖[2] 

机构地区：[1]北京中医药大学,100029 [2]中国中医科学院广安门医院针灸科,北京100053

出　　处：《国际中医中药杂志》2015年第3期235-238,共4页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（81102654）

摘　　要：目的：探讨电针经穴对脑缺血再灌注损伤大鼠海马促肾上腺皮质激素释放因子（corticotropin-releasing factor, CRF）和皮质醇（corticosteroid, CORT）mRNA表达的影响。方法150只SD大鼠按随机数字表法分为经穴电针组、非经穴电针组、模型组、假手术组和正常组各30只。采用改良线栓法制备脑缺血再灌注模型。经穴电针组于脑缺血再灌注后6 h针刺双侧“曲池”“足三里”“百会”“风府”，1次/d，共7 d。脑缺血再灌注后1、3、7 d时评价神经功能缺损后，处死大鼠，取海马组织，利用实时荧光定量PCR检测海马CRF和CORT mRNA表达。结果脑缺血再灌注后1、3、7 d，模型组海马CRF和CORT mRNA[CRF mRNA表达分别为（1.122±0.249）、（1.190±0.666）、（0.454±0.612）， CORT mRNA表达分别为（0.917±0.113）、（1.024±0.290）、（0.709±0.055）]与假手术组[CRF mRNA分别为（0.021±0.049）、（0.021±0.027）、（0.035±0.005），CORT mRNA分别为（0.016±0.013）、（0.016±0.006）、（0.043±0.006）]比较差异有统计学意义（P均＜0.01）。经穴电针组CRF[（0.424±0.104）、（0.339±0.476）、（0.095±0.021）]和 CORT[0.377±0.073）、（0.138±0.025）、（0.158±0.010）]mRNA 表达较模型组显著下降（P均＜0.01）。脑缺血再灌注后1、3、7 d时，经穴电针组神经功能缺损评分为（1.83±0.75）分、（1.50±0.55）分和（1.17±0.41），显著低于模型组的（2.50±0.84）分、（2.33±0.52）和（1.67±0.52）分。结论电针经穴可降低脑缺血再灌注损伤大鼠海马CRF和CORT mRNA表达水平，改善神经功能缺损状态。Objective To investigate the effects of electroacupuncture at meridian point on the mRNA expressions of corticotropin-releasing factor （CRF） and corticosteroid （CORT） in the hippocampus after cerebral ischemia reperfusion in rats.MethodsA total of 150 SD rats were randomly divided into 5 groups by random number table method： normal control group, sham operation group, model group, meridian point electroacupuncture group and non-meridian point electroacupuncture group, with 30 rats in each group. A model of focal cerebral ischemic reperfusion was induced using the modified intraluminal thread method. In the meridian point electroacupuncture group, at 6 h after cerebral ischemia reperfusion, acupuncture “LI11” on both sides, bilateral “ST36”, “GV20”, ”GV16” daily for 7 d. The neurological deficits were evaluated 1 d, 3 d, and 7 d after cerebral ischemia reperfusion, and then all rats were sacrificed and the hippocampi were harvested. The mRNA expressions of CRF and CORT in the hippocampus were determined by quantitative real-time PCR.ResultsThe mRNA expressions of CRF （1.122 ± 0.249, 1.190 ± 0.666, 0.454 ± 0.612 in the model group; 0.021 ± 0.049, 0.021 ± 0.027, 0.035 ± 0.005 in the sham operation group） and CORT （0.917 ± 0.113, 1.024 ± 0.290, 0.709 ± 0.055 in the model group; 0.016 ± 0.013, 0.016 ± 0.006, 0.043 ± 0.006 in the sham operation group） in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly increased in the model group compared with the sham operation group （all P〈0.01）. The mRNA expressions of CRF （0.424 ± 0.104, 0.339 ± 0.476, 0.095 ± 0.021） and CORT （0.377 ± 0.073, 0.138 ± 0.025, 0.158 ± 0.010） in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group （allP〈0.01）. The neurological deficits scale 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the mer

关 键 词：脑缺血 再灌注损伤 电针 促肾上腺皮质素释放激素 糖皮质激素 基因表达 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]