天芪益智颗粒对阿尔茨海默病模型大鼠脑组织氧化应激和学习记忆的影响  

Effects of Tianqi-Yizhi granules on oxidative stress in the brain tissue, and learning and memory in Alzheimer＇s disease model rats

作　　者：赵军 武义明 马涛[3,4] 卫东锋[4] 

机构地区：[1]河北钢铁集团宣化钢铁公司职工医院老年病科,张家口075100 [2]河北钢铁集团宣化钢铁公司职工医院放射科,张家口075100 [3]北京中医药大学东方医院实验中心,100078 [4]北京师范大学老年脑健康研究中心,100875

出　　处：《国际中医中药杂志》2015年第3期239-242,共4页International Journal of Traditional Chinese Medicine

基　　金：国家科技重大专项一重大新药创制（2013ZX09103002-002）;国家自然科学基金重点项目（81430100）

摘　　要：目的：探讨天芪益智颗粒对阿尔茨海默病（alzheimer’s disease, AD）大鼠学习记忆的保护作用及对脑组织氧化应激的影响。方法90只雄性SD大鼠按随机数字表法分为假手术组，模型组，石杉碱甲组以及天芪益智低、中、高剂量组，每组15只。右侧侧脑室注射β-淀粉样多肽1-42（Aβ1-42）制备AD模型。造模1周后开始给药，天芪益智颗粒高、中、低剂量组分别灌胃天芪益智颗粒溶液生药含量分别为0.8、1.6和3.2 g/kg；石杉碱甲组灌胃石杉碱甲溶液0.02 mg/kg；假手术组和模型组灌胃等体积生理盐水。连续灌胃给药30 d。采用避暗实验检测大鼠学习记忆能力。检测脑组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛（MDA）水平。结果与模型组比较，天芪益智颗粒高、中剂量组进入暗室的潜伏期显著延长[（239.05±48.42）s、（214.35±74.52）s比（97.39±30.69）s；P均＜0.01]，错误次数显著减少[分别为（1.93±3.25）次、（2.27±3.09）次比（6.62±3.45）次；P 均＜0.05]，脑组织SOD[（177.27±63.10）U/mg、（164.53±72.58）U/mg比（72.56±21.04）U/mg；P均＜0.01]和 GSH-Px [（2899.36±362.27）U/g、（2407.68±472.14）U/g比（1397.64±442.17）U/g，P均＜0.01]活性显著增高， MDA 水平[（24.75±9.94）nmol/mg、（27.74±5.82）nmol/mg 比（37.56±17.23）nmol/mg，P 均＜0.01]显著降低。结论天芪益智颗粒可减轻AD大鼠脑组织氧化应激，改善学习记忆。Objective To investigate the effects ofTianqi-Yizhi granules on oxidative stress in the brain tissue, and learning and memory in Alzheimer＆#39;s disease model rats.Methods A total of 90 male SD rats were randomly divided into 6 groups by random number table method： sham operation group, model group, huperzine A group and groups of low-, medium- and high-doseTianqi-Yizhi granules, with 15 rats in each group. The AD rat model was prepared by the left lateral ventricle injection of amyloid-β1-42. One week after modeling, the rats in the groups of low-, medium- and high-doseTianqi-Yizhi granules received intragastric administration of 0.8, 1.6 and 3.2 g/kgTianqi-Yizhi granules, respectively; the rats in the huperzine A group received intragastric administration of 0.02 mg/kg huperzine A solution; and the rats in the sham operation and model groups received intragastric administration of equivalent volume of normal saline for 30 days. Learning and memory were evaluated using the dark avoidance test. The activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）, and malonaldehyde （MDA） level in the brain tissue were determined. ResultsIn comparison with the model group, the latencies to step into the dark chamber in the high- and medium-dose Tianqi-Yizhi granules groups were significantly longer （239.05 ± 48.42 s, 214.35 ± 74.52 svs. 97.39 ± 30.69 s; allP〈0.01）, the numbers of errors significantly decreased （1.93 ± 3.25, 2.27 ± 3.09vs. 6.62 ± 3.45; allP〈0.05）, the activities of SOD （177.27 ± 63.10 U/mg, 164.53 ± 72.58 U/mgvs.72.56±21.04 U/mg; all P〈0.01） and GSH-Px （2 899.36 ± 362.27 U/g, 2 407.68 ± 472.14 U/gvs. 1 397.64 ± 442.17 U/g; allP〈0.01）, and MDA level （24.75 ± 9.94 nmol/mg, 27.74 ± 5.82 nmol/mgvs. 37.56 ± 17.23 nmol/mg; allP〈0.01） in the brain tissue significantly increased. ConclusionTianqi-Yizhi granules could attenuate oxidative stress in the brain tissue, and improve learning and memory in AD rats.

关 键 词：阿尔茨海默病 学习 记忆 氧化性应激 大鼠 天芪益智颗粒 

分 类 号：R285.5[医药卫生—中药学]