蛇葡萄素钠协同卡铂体外抑制人肺腺癌GLC-82细胞增值的作用  

The tumor-suppressing effects of ampelopsin sodium combined with carboplatin on human lung adenocarcinoma cell line GLC-82

作　　者：张艳霞[1,2] 缪苗苗[3] 吴勇杰[2] 李文广[2] 

机构地区：[1]甘肃中医学院,兰州730000 [2]兰州大学基础医学院药理学研究所,甘肃省新药临床前研究重点实验室,兰州730000 [3]兰州市第二人民医院,兰州730000

出　　处：《中药药理与临床》2014年第6期38-41,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的：考察蛇葡萄素钠单用及与卡铂合用对人肺腺癌GLC-82细胞增值的抑制作用,初步探讨蛇葡萄素钠抑瘤作用机制。方法：应用MTT法考察蛇葡萄素钠单用及与卡铂合用对人肺腺癌GLC-82细胞的细胞毒作用;使用流式细胞仪检测凋亡相关基因Caspase-3的表达。结果：MTT实验结果表明,蛇葡萄素钠在浓度为12.5~200μg/ml的剂量范围内对GLC-82细胞的增殖有浓度依赖性的抑制作用;卡铂在浓度为3.13~100μg/ml的剂量范围内对GLC-82细胞的增值有浓度依赖性的抑制作用;25、50μg/ml的蛇葡萄素钠对卡铂25μg/ml抑制GLC-82细胞增殖的作用具有协同效应;流式细胞仪检测结果表明,12.5~50μg/ml的蛇葡萄素钠与卡铂25μg/ml联合用药组作用于GLC-82细胞12 h后,Caspase-3表达上调,且有浓度依赖性。结论：蛇葡萄素钠单用对GLC-82细胞的增殖具有一定的抑制作用,与卡铂合用,对GLC-82细胞增殖具有协同抑制效应;蛇葡萄素钠诱导细胞凋亡的机制之一可能是通过激活细胞内的Caspase-3,从而促进凋亡的发生。Objective： To investigate the cytotoxic effect and mechanism of ampelopsin sodium（AMP-Na） on human lung adenocarcionma cell line GLC-82 by alone or combined with carboplatin. Methods： Use the MTT colorimetric method to investigate cytotoxicity effect of AMP-Na a- lone or combined with carboplatin to human lung adenocamionma cell line GLC-82; Use flow cytometry （FCM）analysis the express of Caspase-3. Results： Experimental study on cytotoxicity in vitro showed that GLC-82 cells was inhibited by AMP-Na alone in a concentration- dependent manner ranging from 12.5 ~ 200 μg/ml, and was inhibited by carboplatin alone in a concentration-dependent manner ranging from 3.13 ~ 100 μg/ml. Combined with carboplatin 25 μg/ml, AMP-Na 25 and 50 μg/ml had synergistic effect on the proliferation of GLC-82 cells. Flow cytometry analysis showed that 12 hours after AMP-Na （ 12.5 ~50 μg/ml） combined with carboplatin 25 μg/ml to GLC-82 cell, the express of Caspase-3 up-regulation. Conclusion： AMP-Na had depressant effect to the generation of GLC-82 cell, There is a synergistic cytotoxic effect on GLC-82 cells treated with AMP-Na combined with carboplatin. The mechanisms to induce apoptosis by AMP-Na is proba- bly that activation of Caspase-3 mediated signal transduction pathway in cells.

关 键 词：蛇葡萄素钠 卡铂 人肺腺癌GLC-82细胞 细胞凋亡 Caspase-3 

分 类 号：R285[医药卫生—中药学]