白芍总苷大鼠在体胃肠吸收动力学研究  

Studies on the absorption kinetics of total glucosides of paeony in rats' stomach and intestines

作　　者：韩丽[1] 张玲非[1] 贾春英 谢敏[3] 王鑫国[3] 牛丽颖[3] 

机构地区：[1]河北医科大学第三医院,石家庄050035 [2]正定县人民医院,石家庄050800 [3]河北中医学院中药药理实验室,石家庄050091

出　　处：《中药药理与临床》2014年第6期58-62,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省自然科学基金资助项目(C2010000498)

摘　　要：目的:研究白芍总苷在大鼠体内胃、肠道吸收的动力学特征。方法:采用HPLC色谱法,以芍药苷和芍药内酯苷为指标,测定白芍总苷分别在1 mg/ml、2 mg/ml、4 mg/ml 3个浓度下在体胃吸收情况,白芍总苷3个浓度(其中芍药苷浓度分别为40μg/ml、80μg/ml、120μg/ml,芍药内酯苷浓度14.275μg/ml、28.55μg/ml、42.817μg/ml)的肠循环液在小肠整个肠段的吸收情况,白芍总苷溶液(芍药苷浓度80μg/ml,芍药内酯苷浓度28.55μg/ml)在十二指肠、空肠、回肠、结肠等各肠段的吸收情况。结果:3个浓度白芍总苷在胃内吸收量与药物浓度成正比。白芍总苷3个浓度在小肠内吸收量与药物浓度呈正比,3个浓度吸收速率常数值基本一致。白芍总苷在十二指肠、空肠的吸收量较回肠、结肠大,在十二指肠、空肠吸收速率常数值较回肠、结肠大,在十二指肠、空肠吸收半衰期较回肠、结肠短,经SPSS11.5软件统计分析显示均有显著差异。结论:白芍总苷在胃内吸收可能为被动扩散机制,十二指肠可能为白芍总苷在肠段内吸收主要部位。Objective： To investigate the absorption kinetics of total glucosides of paeony （TGP） in rats＇stomach and intestines. Methods： With paeoniflofin and albiflofin as index, the absorption parameters of TGP at three different concentrations （ 1,2,4 mg/ml） in ratsJstomach in situ perfusion were determined by HPLC, and that of TGP at three different concentrations （including pae？aiflorin 40,80,120 μg/ml and albiflorin 14.275,28.55,42.817 μg/ml） in rats＇small intestine and that of TGP at the concentration （including paeoniflorin 80 μg/ml and albiflorin 28.55 μg/ml） in ratsJduodenum, jejunum, ileum and colon. Results： Absorbed dose of TGP at three concentrations in rats＇stomaeh and small intestine showed direct proportion to drug concentration. The absorption rate constant （Ka） of TGP in small intestine had little variabili-ty under three concentrations. TGP displayed larger absorbed dose, larger Ka and shorter absorbed half llfe period （tl/2） in duodenum and jejunum than ileum and colon, indicating significant deviation by SPSS11. 5. Conclusions： The absorption mechanism of TGP in stomach maybe passive diffusion mechanism. Duodenum maybe the main absorption place of TGP in intestines.

关 键 词：白芍总苷 芍药苷 芍药内酯苷 在体胃肠吸收 吸收动力学 

分 类 号：R285.5[医药卫生—中药学]