当归挥发油对大鼠十二指肠慢波肌电活动的作用研究  

The effect of volatile oil of Angelica sinensis on duodenal slow wave myoelectricity in rats

作　　者：高云娟[1,2] 吴迪[1] 吴国泰[1,3] 杜丽东[1,3] 李应东[1] 文晓玲 任远[1,3] 

机构地区：[1]甘肃中医学院,兰州730000 [2]南充市药品不良反应监测中心,南充637000 [3]甘肃省中药药理与毒理学重点实验室,兰州730000

出　　处：《中药药理与临床》2014年第6期68-71,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金："十二五"国家科技支撑计划项目子课题(2011BAI05B0206);甘肃省自然科学基金项目(1212RJZA079);甘肃中医学院研究生创新基金项目(CX2013-05)

摘　　要：目的:观察当归挥发油对大鼠十二指肠慢波肌电活动的影响。方法:Wistar大鼠禁食不禁水,18h后10%水合氯醛麻醉,手术暴露十二指肠,用银电极自十二指肠垂直纵行肌刺入浆膜下2mm肌层,连接BL-420F生物机能实验系统,以慢波肌电频率(SWMF)和振幅(SWMA)变化为指标,观察当归挥发油对不同诱导剂诱发大鼠十二指肠肌电活动的影响。结果:不同剂量当归挥发油对正常大鼠十二指肠SWMF和SWMA的影响不明显,给药后60min内,与正常对照组比较,组间差异均无统计学意义;0.04mg/kg和0.02mg/kg当归挥发油可拮抗多潘立酮诱导的大鼠十二指肠SWMF和SWMA加强作用,0.01mg/kg当归挥发油可使SWMF加快,而SWMA减弱;0.01mg/kg^0.04mg/kg当归挥发油对新斯的明诱导的十二指肠SWMF加快均有拮抗作用,0.04mg/kg和0.02mg/kg当归挥发油可使SWMA均减弱;0.01mg/kg^0.04mg/kg当归挥发油对氯化钡诱导的十二指肠SWMF加快均有拮抗作用,0.04mg/kg当归挥发油可使SWMA减弱。结论:当归挥发油对正常大鼠十二指肠慢波肌电影响不明显;对肠功能亢进状态的慢波肌电具有抑制作用,作用机制与阻断M受体、阻断钙通道、激动多巴胺受体有关。Objective： To observe the effects of volatile oil of Angelica sinensis on duodenal slow wave myoelectricity in rats. Methods： Wistar rats were fasted for 18h, anesthetized by 10% chloral hydrate, Duodenum was found and silver electrode were respectively placed in muscle lay- er, and then the electrode was connected to the BL-420F biological functional experimental system. The slow wave of myoelectric frequency （SWMF） and amplitude （SWMA） changes were detected. Result： The effects of three different dose of volatile oil of Angelica sinensis （0. 04mg/kg, 0.02mg/kg, 0.01mg/kg） on SWMF and SWMA were not obvious ;The high and middle dose groups （0.04mg/kg, 0.02mg/kg） showed obvious antagonism effect on SWMF and SWMA , but the lower dose groups （0.0 lmg/kg） was contrary （ P 〈 0.01, P 〈 0.05 ） ; The high and middle dose groups （0.04mg/kg, 0. 02mg/kg） showed obvious antagonism effect on SWMF and SWMA changes by domperidone. The three different doses of volatile oil of Angelica sinensis （0.04mg/kg, 0.02mg/kg, 0.01mg/kg） showed obvious antagonism effect on SWMF and SWMA changes by neostigmine and barium chloride. Conclusion： The effects of volatile oil of Angelica sinensis on duodenal slow wave myoelectrieity were not obvious in normal rats, but the inhibited effect were obvious on intestinal hyperfunction state in rats. The mecha- nisms may be related to the blocking of the M receptors and calcium channel blockers, or stimulating dopamine receptors.

关 键 词：当归挥发油 十二指肠 慢波肌电频率 慢波肌电振幅 

分 类 号：R285.5[医药卫生—中药学]