胶球藻多糖对大鼠脑缺血再灌注损伤的保护作用  

Protective effect of Cocoomyxa gloeobotrydifomis on cerebral ischemia-reperfusion injury of rats

作　　者：隋海娟[1] 张玲玲[1] 金英[1] 

机构地区：[1]辽宁医学院药理学教研室,锦州121001

出　　处：《中药药理与临床》2014年第6期118-120,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究胶球藻多糖对线栓法大鼠脑缺血再灌注损伤的保护作用。方法:100只SD大鼠随机分为假手术组、模型组、尼莫地平(1 mg/kg)组、胶球藻多糖(50 mg/kg)剂量组、胶球藻多糖(100 mg/kg)剂量组。采用线栓法建立大鼠脑缺血再灌注模型,用Longa's法、TTC染色法评价大鼠的神经功能评分和脑梗死体积;检测脑组织中一氧化氮合酶(NOS)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)的含量,ELISA法检测对脑组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β水平的影响。结果:与假手术组相比,模型组大鼠神经功能症状、梗死范围明显增高,降低SOD活力,提高MDA,NO,NOS,LDH水平,促进TNF-α和IL-1β的表达。与模型组相比,胶球藻多糖(50 mg/kg)剂量组、(100 mg/kg)剂量组及尼莫地平(1 mg/kg)组可显著降低脑缺血再灌注大鼠的神经功能评分,减轻脑组织损伤程度,减少脑梗死范围,提高SOD活力,降低MDA,NO,NOS,LDH水平,抑制TNF-α和IL-1β的表达。结论:胶球藻多糖对大鼠缺血再灌注损伤具有明显的保护作用,其机制可能与改善神经功能、减少自由基损伤和抑制炎症因子表达有关。Objective： To study the protective effect of Cocoomyxa gloeobotrydifomis on the cerebral ischemia-reperfusion injury of senile rats. Methods： Totally 100 Sprague-Dawley （SD） rats were randomly divided into the sham operation group, the model group, the nimodipine （1 mg/kg） group and Cocoomyxa gloeobotrydifomis （50 mg/kg） doses groups, Cocoomyxa gloeobotrydifomis （ 100 mg/kg） doses group. The rat brain isehemia-reperfusion model was established by the middle cerebral artery occlusion （MCAO） method in rats, the infarct volume and the neurological deficit were determined by the TTC staining and Longag score; And determine the contents of NOS, NO, SOD, MDA and LDH in brain tissues. The contents of TNF-ot and IL-113 levels in brain tissues were measured by enzyme-linked immunosorbent assay （ELISA） method. Results： Compare with the sham operation group, model group rats significantly increased infarct volume, the extent of edema, and aggravated the neurological deficit, decrease the vigor of SOl3, increase the levels of MDA, NO, NOS and LDH, and improve TNF-a and IL- 1β expressions. Compare with the model group, Cocoomyxa gloeobotrydifomis （50 mg/kg） doses groups, （100 mg/kg） doses groups and ni- modipine （1 mg/kg） group could significantly decrease neurological score, injury degree of brain tissues and brain infarct volume of rats with cerebral ischemia-reperfusion injury, increase the vigor of SOD, decrease the levels of MDA, NO, NOS and LDH, and inhibit TNF-a and IL-1β expressions. Conclusion： Cocoomyxa gloeobotrydifomis has the obvious＇ protective effect on the brain ischemia-reperfusion injury in rats. Its mechanism may be related to the improvement of neurological function, the reduction of free radical injury, and the inhibition of in- flammation factor expression.

关 键 词：胶球藻多糖 脑缺血再灌注损伤 自由基 炎症因子 神经功能 白细胞介素-1 

分 类 号：R285.5[医药卫生—中药学]