嘎日迪-13对脑缺血后大鼠不同时相NSE、TNF-α、IL-8表达的影响  

Effect of Gridi-13 on the expression of NSE,TNF-α and IL-8 at different phase after focal cerebral ischemia in rats

作　　者：田彩云[1] 张浩楠[1] 何静波[1] 武海军[1] 覃建民[1] 徐继辉[1] 杨玉梅[1] 

机构地区：[1]包头医学院心血管研究室,内蒙古包头014040

出　　处：《中药药理与临床》2014年第6期158-161,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目;编号81160560

摘　　要：目的:探讨嘎日迪-13对局灶性脑缺血大鼠的保护作用。方法:雄性SD大鼠360只,将大鼠随机分为脑缺血假手术组、模型组、嘎日迪-13 240mg/kg组、嘎日迪-13 120mg/kg组、嘎日迪-13 60mg/kg五个组,每组又随机分为缺血1 h组、6 h组、12 h组、24 h组、72 h组与120 h组6个时相组,采用改良Zea Longa线栓法制备大鼠大脑中动脉栓塞模型,ELISA法检测血清中神经元特异性烯醇化酶(neuron specific enolase,NSE)和脑组织中TNF-α、IL-8含量。结果:与模型组比较,嘎日迪-13(240、120和60mg/kg)组显著降低Zea Longa评分,减少血清中NSE含量,不同程度的降低了脑组织中TNF-α、IL-8表达。结论:嘎日迪-13对脑缺血损伤大鼠有一定的神经保护作用,可能与其有效的降低血清中NSE的水平,抑制炎症因子TNF-α、IL-8的表达有关。Objective： Investigating the protective effect of Gridi-13 on the focal cerebral ischemia injury with rats. Methods： A total of 360 male SD rats were selected. They were randomly divided into model group, sham-operated group, Gridi-13 240mg/kg group, Gridi-13 120mg/kg group and Gridi-13 60mg/kg group. Each group was randomly divided into 1 h, 6 h, 12 h, 24 h, 72 h and 120 h group. The middle cerebral artery occlusion （MCAO） model was established by modified Zea Longa thread-occlusion method and the content of neuron specific enolase （NSE） in serum, TNF-a and IL-8 in brain tissue was determined by the ELISA method. Results： Compared with the model group. 240, 120,60mg/kg of Gridi-13 could predominantly reduce the Zea Longa score and the expression levels of NSE in serum （P 〈 0.01 ） ,the TNF- a and the IL-8 in brain tissue were remarkably inhibited at different degrees （ P 〈 0.01 or P 〈 0.05 ）. Conclusion： Garidi-13 has protective effect on cerebral ischemia injury which may be related to remarkably reduce the level of NSE in serum and inhibit the inflammatory factors of TNF-a, IL-8 in brain tissue.

关 键 词：嘎日迪-13 脑缺血 神经元特异性烯醇化酶 TNF-Α IL-8 

分 类 号：R29[医药卫生—民族医学]