高压氧暴露对卵巢早衰大鼠卵巢、子宫及血清雌二醇的影响  

Effects of hyperbaric oxygen on ovary, uterus and 17-estradiol in rats with premature ovary failure

作　　者：王春莲[1] 郝娟 邱丽丽[1] 万金娥[3] 闫丽萍[1] 王培嵩[3] 

机构地区：[1]青岛大学附属医院妇科,山东省青岛266003 [2]青岛市妇女儿童医院,山东省青岛266003 [3]青岛大学附属医院高压氧科,山东省青岛266003

出　　处：《中华航海医学与高气压医学杂志》2014年第3期145-149,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：青岛市科技计划基础研究项目（09-1-3-81-jch）;青岛市科技计划项目（08-2-1-5-nsh）

摘　　要：目的 探讨高压氧（HBO）暴露对药物致卵巢早衰大鼠的影响.方法 50只SPF级雌性Wistar大鼠按数字表法随机分为5组：正常组、模型组、对照组、雌孕激素替代治疗（HRT）组和HBO组,每组10只.除了正常组外,其余各组均给予雷公藤多甙片溶液灌胃,建立卵巢早衰模型.正常组和模型组大鼠在灌胃14 d后分别留取血清,处死并留取卵巢和子宫.其余各组给予不同干预,于造模后40 d留取血清,处死并留取卵巢和子宫.同批检测血清雌二醇（E2）水平,并对其动情周期、子宫和卵巢的脏器指数、子宫内膜厚度、卵巢面积等进行观察分析.结果 灌药后,大鼠动情周期发生紊乱,表现形式多样;各组给予干预后,其动情周期均不同程度逐渐恢复正常,对照组动情周期仍表现紊乱;模型组血清E2水平、内膜厚度以及脏器指数均低于正常组,单位卵巢面积内卵泡数较正常组少（P＜0.05）,闭锁卵泡数较正常组多（P＜0.05）;卵巢面积较正常组小;干预40 d后,HBO组血清E2水平较HRT组和对照组均高（均P＜0.05）.HBO组和HRT组子宫内膜厚度和子宫脏器指数较对照组高,HBO组较HRT组高（均P ＜0.05）.HBO组单位卵巢面积、卵泡数比HRT组和对照组多,但仅与对照组的差异有统计学意义（P＜0.05）.HBO组和HRT组闭锁卵泡数比对照组少（P＜0.05）.结论 雷公藤多甙片灌胃法建立大鼠卵巢早衰模型是可行的;HBO暴露可以改善卵巢功能,使E2水平升高,增加子宫、卵巢的重量及子宫内膜厚度.Objective To investigate the effects of hyperbaric oxygen （HBO） on rats with premature ovary failure （POF） induced by triptergium wilfordii polyglycisidium （TWP）.Methods Fifty female SPF rats were randomly divided into 5 groups：the normal group,the model group,the control group,the hormone replacement therapy （HRT） group and the HBO group,each consisiting of 10 animals.Except the normal group,all the other groups were given orally triptegium wilfordii polyglycisidium （TWP） to establish premature ovary failure model.Fourteen days after intragastric administration of TWP,serum samples from the normal and model groups were taken,then the animals were sacrificed and uterus and ovary were collected for detection.The animals in other groups were treated differently,and 40 days after establishment of the model,serum samples were taken,then the animals were also sacrificed and uterus and ovary were collected for the detection of related parameters.Serum estradiol （E2） level,changes in estrous cycle,uterine and ovarian weight,endometrial thickness and ovarian size were observed and analyzed.Results After intragastric administration of the drug,estrous cycle of rats was disturbed in various forms.Following treatment with HRT and HBO,estrous cycle all gradually recovered to a more or less extent,while estrous cycle of the rats in the control group still remained in a state of disorder.Serum E2 level,endometrial thickness and organ weight index in the model group were significantly lower than those of the normal group （P 〈 0.05）.Besides,follicular number in ovary unit area was significantly less than that of the normal group,while atretic follicular number was obviously more than that of the normal group （P 〈 0.05）.Ovary area in the model group was smaller than that of the normal group （P 〉 0.05）.Forty days after intervention,serum E2 level in the HBO group was higher than that of the control group and the HRT group （P 〈 0.05）.Endometrial thickness and uterus weight indexes i

关 键 词：卵巢早衰 高压氧 激素替代治疗 雌二醇 大鼠 

分 类 号：R711.7[医药卫生—妇产科学]