高压氧对大鼠行为学及应激相关指标影响的研究  

Research on the effects of hyperbaric oxygen on rats behavior and stress-related indicators

作　　者：麦乃铿 杨晨[2] 孙瑞佼 吕艳[2] 赵津京 潘树义[2] 

机构地区：[1]南方医科大学研究生院,广州510515 [2]海军总医院高压氧科 [3]解放军第三○五医院神经内科

出　　处：《中华航海医学与高气压医学杂志》2014年第6期365-368,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的 通过对大鼠行为学及应激相关指标的观察,探讨高压氧与应激的关系.方法 20只Wistar大鼠按照数字表法随机分为高压氧组和空白对照组,高压氧组给予0.20 MPa高压氧处理,连续治疗3周;对照组单纯喂养,分别在第1、7、14、21天对其进行高架十字迷宫试验,观察大鼠开臂逗留时间,利用酶联免疫吸附测定法测定其血清中促肾上腺皮质激素释放激素、促肾上腺皮质激素、糖皮质激素、一氧化氮合酶、一氧化氮及肿瘤坏死因子-α（TNF-α）含量.结果 高压氧处理可明显增加大鼠外周血液中糖皮质激素（glucocorticoids,GCS）的水平,差异具有统计学意义（P＜0.05）,但对大鼠高架十字迷宫中的开臂逗留时间、一氧化氮系统及TNF-α的表达无明显影响（P＞0.05）.结论 高压氧处理符合慢性应激的一般特征,但又不对机体造成明显的不良影响,提示高压氧的作用机制之一是作为一种良性应激来增加机体对应激的耐受能力.Objective To investigate the relationship between hyperbaric oxygen （HBO） and stress through observation on rats behavior and stress-related indicators.Methods Twenty Wistar rats were randomly divided into the HBO group and the control group.The HBO group was exposed to HBO at a pressure of 0.20 MPa once a day,for a succession of 21 days,and the control group was left there without HBO treatment.After one week of adaptive training,elevated plus maze test was conducted at days 1,7,14 and 21 to observe openarm （OA） time.The contents of corticotropin-releasing hormone （CRH）,adrenocorticotropic hormone （ATCH）,glucocorticoids （GCS）,nitric oxide synthase （NOS）,nitric oxide （NO） and tumor necrosis factorα （TNF-α） in serum of the animals in each group were detected by ELISA （enzyme-linked immunosorbent assay）.Results HBO treatment could significantly increase the level of GCS in peripheral blood （P ＞ 0.05）,but with no obvious effect on open-arm time （anxiety degree） and the expression levels of nitric oxide and TNF-α.Conclusions HBO treatment was in conformity with the general characteristics of chronic stress,but would not bring about damage to the body,suggesting that one of the mechanisms of HBO might lie in the increased tolerance of the involved tissues to stressors.

关 键 词：高压氧 高架十字迷宫 HPA轴 一氧化氮系统 肿瘤坏死因子-Α 大鼠 

分 类 号：R459.6[医药卫生—治疗学]