趋化因子受体4在高压氧致肺型氧中毒大鼠模型肺组织中含量的变化  

Change pattern for the expression of chemokine receptor 4 in the lung tissue of rats with pulmonary oxygen toxicity

作　　者：张师[1] 方以群[1] 李开诚[1] 包晓辰[1] 攸璞[1] 王海涛[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《中华航海医学与高气压医学杂志》2014年第6期387-389,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：海军医学研究所所基金课题（编号：12HY12）;海军后勤部卫生部课题（BW511J053）;海军后勤部卫生部课题（BHJ12J003）

摘　　要：目的 研究肺型氧中毒（pulmonary oxygen toxicity）大鼠肺组织损伤中趋化因子受体4（chemokine receptor4,CXCR4）的变化规律.方法 雄性健康SD大鼠30只,体质量200～ 220 g,按数字表法随机分为6组,每组5只.其中1组为常压空气对照组,该组大鼠暴露于自然大气环境中,禁食水,不进行任何加减压实验处理;另外5组分别在高压纯氧环境下暴露2、4、6、8、10 h后逐组出舱进行动物存活实验动物处理.实验动物氧舱底铺设新鲜钠石灰,100％氧气持续灌舱10 min,氧流量计测定舱内氧浓度达99％后,以0.1 MPa/min的速率匀速加压至0.23 MPa.持续微量通风,维持舱内氧浓度＞99％.观察各组大鼠肺组织病理改变,应用蛋白免疫印记法检测CXCR4,并与常压空气对照组对比.结果 随着氧暴露时间增加,肺组织出现淤血、水肿,出血程度不断加重.CXCR4表达在8h内随时间增加而增高,但在10h组表达有所下降.结论 高压纯氧暴露后大鼠发生了肺脏组织损伤,与CXCR4变化规律基本一致.Objective To study the change pattern for the expression of chemokine receptor 4 （CXCR4） in the lung tissue of rats with pulmonary oxygen toxicity.Methods Thirty SD rats were randomly divided into 6 groups：the control group,the 2 h pure oxygen exposure group （or simply the 2 h group）,the 4 h pure oxygen exposure group （the 4 h group）,the 6 h group,the 8 h group and the 10 h group,each consisting of 5 animals.Observations on pathological changes in the lung tissue and changes in the CXCR4 expression levels in the lung tissues of rats were performed by Western Blot,then,comparisons were made with those of the control group.Results Pathological detection indicated that stasis of blood,edema and hemorrhage in the alveolar and pulmonary interstitial tissue deteriorated with the extension of oxygen exposure time.The expression level of CXCR4 in the lung tissue increased with the extension of exposure within 8 hours,however,the levels of CXCR4 in the 10 h group decreased to some extent,as compared with that of the 8 h group.Conclusions Mter exposure to high pressure oxygen,lung damage occurred in pulmonary tissue of rats,with the change pattern basically identical to that of CXCR4.

关 键 词：趋化因子受体4 氧中毒 肺组织 大鼠 

分 类 号：R84[医药卫生—航空、航天与航海医学]