二尖瓣狭窄动物模型的建立  

Establishment of Animal Model of Mitral Stenosis

作　　者：张浒[1] 陶杰[1] 白向锋[1] 段冰松[1] 孙毅[1] 李丽娜[1] 张桂敏[1] 

机构地区：[1]昆明医科大学第一附属医院心血管外科,云南昆明650032

出　　处：《昆明医科大学学报》2015年第2期1-3,共3页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(31360227)

摘　　要：目的探讨建立猪二尖瓣狭窄实验模型的可行性.方法选择质量42-56 kg的大型猪6头,采用全麻体外循环（cardiopulmonary bypass,CPB）下经左心耳入路,4-0无创缝线分别于二尖瓣A1-P1区和A3-P3区水平褥式缝合.结果共制备出猪二尖瓣狭窄模型6个,总成功率100%,心脏超声提示二尖瓣口的血流速度、跨瓣压差及瓣口面积手术前后比较差异有统计学意义（P〈0.05）.结论大型猪制备二尖瓣狭窄动物模型,科学可靠,可行性强,成模率高,可用于心瓣膜疾病发病机制的研究.Objective To assess the feasibility of establishing the model of mitral stenosis in swine.Methods Six swines（42-56 kg） were performed operation in which the the anterior leaflet and posterior leaflet were sutured at A1-P1 and A3-P3 through the left atrial appendage route under the cardio pulmonary bypass and general anesthesia. Results A totally 6 swine models of mitral stenosis were made successfully,the success rate was 100%. There were significant differences in the blood flow velocity,mitral pressure of gradient and effective orifice area between pre-operation and post-operation.Conclusions The method of preparing mitral stenosis model in swine is simple,scientific,and effective with good feasibility and satisfying success rate.The model can be used for reseaching the pathogenesis of rheumatic heart disease

关 键 词：二尖瓣狭窄 体外循环 动物模型 

分 类 号：R332[医药卫生—人体生理学]