滇南小耳猪深Ⅱ度烧(烫)伤模型的建立  

Establishment of the Deep Second Degree Burn Models in Diannan Xiaoer Pigs

作　　者：刘彦[1] 郭徽[1] 缪玉兰[2] 胡冬雄[1] 张幻诗[1] 钱忠义[3] 杨建宇[1,3] 

机构地区：[1]昆明医科大学海源学院,云南昆明650106 [2]昆明医科大学第二附属医院,云南昆明650101 [3]昆明医科大学基础医学院,云南昆明650500

出　　处：《昆明医科大学学报》2015年第2期10-14,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学联合专项基金资助项目(2011FB246);云南省科技厅应用基础研究基金资助项目(2011FZ126)

摘　　要：目的探索滇南小耳猪背部皮肤深Ⅱ度烧（烫）伤模型的制作方法.方法分别使用混合燃料和高温铜块在不同时间内对滇南小耳猪背部皮肤进行致伤,取烧（烫）伤创面组织进行苏木精-伊红对染法（HE染色）,观察不同致伤时间猪背部皮肤的烧（烫）伤深度,从而建立猪背部深Ⅱ度烧（烫）伤模型.结果混合燃料及高温铜块在不同致伤时间内可造成不同深度的烧（烫）伤.而使用混合燃料烧伤30 s或高温铜块烧（烫）伤70 s可以造成猪深Ⅱ度烧（烫）伤模型,成功率达94.4%,95%CI为72.71%-99.86%.结论使用混合燃料烧伤30 s或高温铜块烧伤70 s可以成功建立猪深Ⅱ度烧（烫）伤模型.Objective To explore the methods of establishing the deep second degree burn models in the Dian Nan Xiao Er pigs. Methods The Dian Nan Xiao Er pigs were injured by the mixed fuel or the heat copper blocks at different times on the back. Then the tissues from the burn wounds were drawn and dyed with hematoxylin-eosin. All sections were observed and estimated by the depth of burn injury,Therefore, the deep second degree burn models in the Dian Nan Xiao Er pigs were successfully built. Result The mixed fuel or the heat copper blocks could lead to different degree burn.Applications of the mixed fuel flamed in 30 seconds or the heat copper blocks scalded in 70 seconds can both lead to the deep second degree burn in the Dian Nan Xiao Er pigs with a success rate of 94.4%,and95%CI of 72.71%-99.86%.Conclusion Applications of the mixed fuel flamed in 30 seconds or the heat copper blocks scalded in 70 seconds can both successfully build the deep second degree burn models in the Dian Nan Xiao Er pigs.

关 键 词：滇南小耳猪 混合燃料 高温铜块 深Ⅱ度烧(烫)伤模型 

分 类 号：R644[医药卫生—外科学]