螺蛳分子系统发育的初步研究  

Studies On Molecular Phylogeny of Margarya melanioides(Gastropoda:Viviparidae)

作　　者：田明[1] 范彪[1] 杨瑜玲[1] 方福康[1] 王文林[2] 陈元晓[1] 

机构地区：[1]昆明医科大学细胞生物学与医学遗传学系,云南昆明650500 [2]昆明医科大学病原生物学系,云南昆明650500

出　　处：《昆明医科大学学报》2015年第3期4-8,33,共6页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(31160425;31101616;81360252);云南省科技厅应用基础研究基金项目(2010ZC108);云南省教育厅科学研究基金资助项目(2010Y208)

摘　　要：目的建立田螺科已有种类的系统发育关系,探讨螺蛳(Margarya melanioides)的分类地位.方法测定采自云南高原湖泊中的螺蛳、方形环棱螺和中华圆田螺的线粒体COI、16S r RNA基因序列,并结合Gen Bank中田螺科已有物种序列,计算螺蛳和其他主要类群的遗传距离,用最大简约法(MP)、最大似然法(ML)和贝叶斯法(BI)构建包含螺蛳的田螺科部分物种系统发育树.结果 COI基因第三位密码子转换已达到饱和.在COI基因片段数据集、COI第1,2位密码子数据子集、16S基因片段数据集中,螺蛳与圆田螺属的遗传距离最小.在MP法、ML法、BI法构建的系统发育树中,螺蛳和中华圆田螺聚为一支,我国的环棱螺属种类和非洲该属未聚在一起.结论螺蛳与中华圆田螺的亲缘关系较近,线粒体COI联合16S基因片段可以弥补形态学在研究田螺科物种系统发育关系时的不足.Objective To infer the phylogenetic relationship among Viviparidae, and to assess the phylogenetic position of M. melanioides. Methods Sequence mitochondrial COI and 16 s gene of M. melanioides,Bellamya quadrata and Cipangopaludina cathayensis collected from Yunnan plateau of China were tested. Kimura-2parameters including genetic distance and Ts/Tv ratios were generated,and the molecular phylogenetic trees of 26 species in Viviaprdae, including M. melanioides, were established by using Maximum likelihood（ML）,maximum parsimony（MP） and Bayesian inference（BI） methods.Results The 3rd codon position of COI,which get high saturation, had negative impact on phylogenetic anaysis. The nearest genetic neighbor of M.melanioides in Viviparidae was C. cathayensis among DNA sequence set with COI, or COI-1st and COI-2nd codon positons,or 16 S gene.In using MP, ML and BI methods,M. melanioides and C. cathayensis form one phylogenetic clade,but Bellamya species from china and Africa were in different phylogenetic units. Conclusion C. cathayensis is closest to M. melanioides,and molecular data of COI and 16 S gene could cover shortage of phylogenetic research based on morphological characters in viviparidae.

关 键 词：螺蛳 田螺科 分子系统发育 16S COI 

分 类 号：Q179[生物学—水生生物学]