IL-4基因启动子SNP-1098T>G和-590C>T多态性与云南汉族人群HCV慢性感染的相关性研究  

Association of SNP-1098T>G and-590C>T in IL-4 Gene Promoter with Chronic HCV Infection in Yunnan Han Population

作　　者：戴书颖[1] 戴书朋[2] 喻箴[3] 张才军 吴虢东[1] 宝福凯[1] 

机构地区：[1]昆明医科大学基础医学院病原生物学与免疫学系,云南昆明650500 [2]昆明医科大学第一附属医院肝胆外科,云南昆明650032 [3]昆明医科大学公共卫生学院流行病与卫生统计学系 [4]基础医学院病原生物学与免疫学实验中心,云南昆明650500

出　　处：《昆明医科大学学报》2015年第3期9-12,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81060134;81371835);云南省自然科学基金资助项目(2010CD221;2011FB244;2012FB011;2013FZ057;2014FA011;2014FB001)

摘　　要：目的探讨IL-4基因启动子区SNP-1098T>G和-590C>T位点与HCV慢性感染的相关性.方法采用Taq Man探针基因分型方法对HCV慢性感染患者192例和健康体检人群170例IL-4基因启动子SNP-1098T>G和-590C>T位点进行基因分型,计算2个SNP位点的连锁不平衡,并构建单倍型,获得以上2个SNP位点及其单倍型与HCV慢性感染的相关性数据.结果 IL-4基因启动子SNP-1098T>G和-590C>T位点基因型和等位基因频率分布差异在病例组和对照组中无统计学意义(P>0.05).连锁不平衡结果显示,SNP-1098T>G和-590C>T位点为连锁不平衡,D’值为0.937.构建单倍型后发现,SNP-1098T>G和-590C>T位点构建的单倍型频率分布差异在病例组和对照组中无统计学意义(P<0.05).结论 IL-4基因启动子区SNP-590C>T和-1098T>G多态性与云南汉族人群HCV慢性感染的无相关性.Objective To study the relationship between the allele polymorphisms SNP-1098T 〉G and -590C 〉T in interleukin-4 gene promoter and HCV chronic infection in Han population in Yunnan province . Methods Two single nucleotide polymorphisms （SNPs） in the IL-4 promoter region, -1098T〉G and -590C 〉T in 192 HCV chronic infected patients and 170 healthy person were determined by real-time TaqMan polymerase chain reaction. We calculated the linkage disequilibrium of the two SNPs, and then constructed haplotypes, obtained correlative data between SNP-1098T 〉G, -590C 〉T and HCV chronic infection, and the correlation between haplotype frequency and HCV chronic infection. Results IL4-1098T 〉G and -590C 〉T genotype and haplotypes frequency showed no significant association with HCV infected patients and healthy person （P〉0.05） There was linkage disequilibrium of IL-4 gene between SNP-1098T〉 G and -590C 〉T. The frequency of haplotypes constructed by SNP-1098T〉G and -590C 〉T showed no significant difference between HCV infected patients and healthy person （P 〉0.05） Gonclu sion SNPs-1098T 〉G and -590C 〉T in IL-4 gene promoter has no association with chronic HCV infection in Yunnan Han population.

关 键 词：HCV 单倍型 IL-4 单核苷酸多态性 

分 类 号：R392[医药卫生—免疫学]