添加晶体或包膜DL-蛋氨酸对利用豆粕替代花鲈饲料中鱼粉的影响  

Effects of crystalline and capsulated DL-methionine on fish meal replacement with soybean meal in diets for Japanese seabass Lateolabrax japonicus

作　　者：张艳秋[1,2] 纪文秀[3] 吴玉波[1] 王岩[4] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310058 [2]钦州学院海洋学院广西北部湾海洋生物多样性养护重点实验室,广西钦州535000 [3]浙江大学舟山海洋研究中心,浙江舟山316021 [4]浙江大学海洋学院,浙江杭州310058

出　　处：《水产学报》2015年第2期224-232,共9页Journal of Fisheries of China

基　　金：浙江省科技厅项目(2008C22055);国家自然科学基金(30771673)

摘　　要：通过8周生长实验检验添加晶体或包膜DL-蛋氨酸对利用豆粕替代花鲈饲料中鱼粉的影响,以确定添加DL-蛋氨酸对提高饲料鱼粉替代水平的作用。对照饲料鱼粉水平为40%。采用2×4实验设计,按等蛋白替代原则分别用豆粕替代对照组饲料中鱼粉的40%(L)和80%(H);在每个鱼粉替代水平上,分别添加晶体DL-蛋氨酸(A)、包膜DL-蛋氨酸(B)、晶体DL-蛋氨酸和包膜材料(C)以及按1∶1比例配制的B和C的混合物(D)。配成8种等氮、等脂肪的实验饲料(LA、LB、LC、LD、HA、HB、HC和HD)。饲料LA、LB、LC和LD含24%鱼粉,并分别添加0.5%A、1.3%B、1.3%C或1.3%D;饲料HA、HB、HC和HD含8%鱼粉,并分别添加0.7%A、1.8%B、1.8%C或1.8%D。实验鱼初始体质量为(6.0±0.1)g。实验结果表明,饲料鱼粉替代水平显著影响花鲈增重(WG)、摄食率(FI)、饲料系数(FCR)、饲料氮沉积效率(NRE)、饲料氮废物排放量(TNW)、肝体比(HSI)以及全鱼水分和粗脂肪含量;DL-蛋氨酸剂型可显著影响饲料磷废物排放量(TPW)。当添加的DL-蛋氨酸剂型相同时,WG和NRE随饲料鱼粉替代水平增加而下降,而FCR和TNW增加;在相同饲料鱼粉替代水平下,添加晶体或包膜DL-蛋氨酸未导致花鲈WG、FCR、NRE、CF、HSI、鱼体组成、TNW和TPW出现显著差异。上述结果显示,通过添加豆粕可将花鲈饲料中鱼粉含量降低至24%,而添加晶体或包膜DL-蛋氨酸不能进一步提高利用豆粕替代饲料鱼粉的水平。An 8-w eek feeding trial was conducted to evaluate the effects of crystalline and capsulated DLmethionine on fish meal replacement with soybean meal in diets for Japanese seabass Lateolabrax japonicus.A reference diet（control） was formulated to contain 40% fish meal.A 2 × 4 layout was used,including two fish meal replacement levels（40% or 80% of the fish meal in control diet were replaced with soybean meal）and four types of DL-Met supplementation（A：Crystalline DL-Met;B：Capsulated DL-Met;C：Crystalline DL-Met ＋ capsule material;D：a premix containing B and C at 1 ∶ 1）.The 8 test diets were formulated isonitrogenous and isolipidic.0.5% A,1.3% B,1.3% C or 1.3% D were added in diets LA,LB,LC and LD（dietary fish meal level was 24%）,respectively,while 0.7% A,1.8% B,1.8% C or 1.8% D were added in diets HA,HB,HC and HD（dietary fish meal level was 8%）.Initial body weight of Japanese seabass was（6.0 ± 0.1） g.Fish meal replacement level significantly affected weight gain（WG）,feed intake（FI）,feed conversion ratio（FCR）,nitrogen retention efficiency（NRE）,total nitrogen wastes output（TNW）,hepatosomatic index（HSI） and proximate composition of whole fish（P 〈 0.05）;while types of DL-Met affected total phosphorus wastes output（TPW）（P 〈 0.05）.The WG and NRE decreased（P 〈0.05）,while the FCR and TNW increased,with increasing of fish meal replacement level,whether crystalline or capsulated DL-Met was added.At the same fish meal replacement level,no significant differences were found in the WG,FCR,NRE,CF,HSI,body composition,TNW and TPW betw een fish fed the diets with supplementation of A,B,C or D（P 〉 0.05）.This study indicates that dietary fish meal level for Japanese seabass can be reduced to 24% when SBMwas used as a fish meal substitute,and supplentation of crystalline or capsulated DL-Met could not enhance fish meal replacement level by SBMin diets for Japanese seabass.

关 键 词：花鲈 豆粕 DL-蛋氨酸 生长 饲料利用效率 

分 类 号：S963.7[农业科学—水产养殖]