过表达枸杞LcCHYB基因提高洋桔梗抗氧化性的研究  

Overexpression of Lc CHYB to Enhance the Tolerance to Oxidative Stress of Eustoma Grandiflorum

作　　者：金超[1] 柳洁[1,2] 季静[1] 王罡[1] 曹海燕[1,2] 吴疆[1,2] 

机构地区：[1]天津大学环境科学与工程学院,天津300072 [2]天津大学化工学院,天津300072

出　　处：《中国生物工程杂志》2015年第1期27-33,共7页China Biotechnology

基　　金：国家自然科学基金(31300329;31271793;31271419);国家转基因生物新品种培育科技重大专项(2014ZX08003-002B)资助项目

摘　　要：非生物胁迫造成植物胞内产生大量活性氧ROS和自由基,导致植物细胞结构及酶系统受到破坏。类胡萝卜素是植物胞内重要的抗氧化物质,与植物的抗氧化功能有重要关系。为了提高洋桔梗的抗氧化性,将来自枸杞(Lycium Chinense Miller)的β-类胡萝卜素羟化酶基因(Lc CHYB)导入洋桔梗中,研究结果表明以10%过氧化氢胁迫洋桔梗叶片20 min后,转基因洋桔梗叶片中总类胡萝卜素、玉米黄质含量及叶黄素循环池容量显著提高,同时对光合荧光参数和抗氧化酶系统的酶活性也进行探讨,发现转基因洋桔梗相对于野生型植株对氧化胁迫的耐受性也有了显著的提高。Reactive Oxygen Species was accumulated when the plant was under the abiotic stress,which damaged the cell structure and proteins. Carotenoids are non-enzymatic antioxidants,playing an important role to enhance the tolerance to oxidative stress in plants. β-carotene hydroxylase was a key enzyme in carotenoid biosynthesis pathway and coded by CHYB gene. In this study,the Lc CHYB was overexpressed in transgenic Eustoma grandiflorum,the results shown When the plants were exposed to 10% H2O2,the Fv/ Fm,ФPSⅡ,SOD and POD increased significantly,the content of total carotenoids,Zeaxanthin and Xanthophyll cycle were also enhanced significantly in transgenic line compare to the wild type. Overexpression of Lc CHYB enhanced the tolerance to oxidative stress in Eustoma grandiflorum was proved.

关 键 词：洋桔梗 β-类胡萝卜素羟化酶 氧化胁迫 

分 类 号：Q819[生物学—生物工程]