酞酸酯DMP、DEP和DBP对明亮发光杆菌的毒性研究  

Study on Toxicity of Phthalate Acid Esters(PAEs) to Photobacterium phosphoreum

作　　者：李海燕[1] 王平[1] 刘雯[1] 王心春[1] 尉腾达 

机构地区：[1]南京林业大学生物与环境学院,江苏南京210037

出　　处：《生态环境学报》2015年第1期121-125,共5页Ecology and Environmental Sciences

基　　金：国家自然科学基金面上项目(31270680);江苏高校优势学科(环境科学与工程)建设工程资助项目

摘　　要：酞酸酯（PAEs,phthalate acid esters）是目前最普遍的有机污染物之一,一定剂量的酞酸酯会产生生殖和发育毒性等健康危害。以明亮发光杆菌（Photobacterium phosphoreum T3）为受试生物,评价了甲醇和3种PAEs[dimethyl phthalate（DMP）,diethyl phthalate（DEP）,dibutyl phthalate（DBP）]对其剂量—效应关系,以期为酞酸酯暴露风险评价提供一定理论依据。结果表明,采用Logit和Weibull非线性函数（全浓度区间）分别对甲醇和3种PAEs的浓度—效应曲线进行拟合,效果均优于线性函数（局部浓度区间）拟合。其中,甲醇的线性与Logit函数拟合r^2分别为0.959 2和0.986 3,两者EC50分别为1.185和1.044 mol·L^-1,结果相近。数据结果表明,采用明亮发光杆菌测试PAEs急性毒性时,以甲醇为助溶剂（浓度〈0.247 mol·L^-1,毒性效应可忽略）是可行的。在一定浓度范围内,首次以甲醇为助溶剂,以DMP、DEP和DBP 3种PAEs对明亮发光杆菌进行毒性测试。线性拟合DMP、DEP和DBP对明亮发光杆菌的浓度-效应曲线,拟合r^2分别为0.926 2、0.921 4、0.933 9,其EC50分别为2.64E-04、2.79E-04和1.14E-05 mol·L^-1。与此同时,3组PAEs毒性数据均能以Weibull函数进行非线性拟合,r^2分别为0.974 9、0.974 2和0.964 8,效果良好,其EC50分别为1.49E-04、2.72E-04和9.00E-06 mol·L^-1。两种拟合结果表明,Weibull函数非线性拟合效果更优,半致死浓度EC50结果相近。由上述结果可知,以甲醇为助溶剂时,DMP、DEP和DBP分别在6.14E-9-6.14E-3、5.04E-5-2.52E-3和3.76E-9-3.76E-5 mol·L^-1浓度范围内,3种PAEs对明亮发光杆菌的急性毒性强弱为DBP〉DMP〉DEP。在上述浓度范围内,DMP、DEP和DBP对明亮发光杆菌的抑制率分别为96.87%、95.24%和88.35%。Phthalate acid esters（PAEs） are one of the most common organic pollutants currently. A dose of PAEs would bring about health hazards such as reproductive and developmental toxicity. To provide some academic evidence of exposure and risk assessment to PAEs, the dose-response of methanol and the three PAEs（DMP, DEP and DBP） to Photobacterium phosphereum T3 was evaluated. The results showed that, the dose-response of methanol and the three PAEs could be separately better non-linear fitted by Logit and Weibull equations（in full concentration range） compared to linear fitting（part of the concentrations）. For methanol, the correlation coefficients R^2 of linear fitting and non-linear fitting were 0.959 2 and 0.986 3 separately. And their EC50 calculated by both ways were similar with results of 1.185 and 1.044 mol·L^-1. The data results showed that it was feasible for methanol（with concentration of 0.247 mol·L^-1） to act as co-solvents for the three PAEs（DMP, DEP and DBP） in acute toxicity test. The toxicity assessment of the three PAEs to Photobacterium phosphoreum T3 was performed in certain doses. Linear fitting was executed for the dose-response curves of DMP, DEP and DBP. Their EC50 values were 2.64E-04, 2.79E-04 and 1.14E-05 mol·L^-1, while the three correlation coefficients r^2 were 0.926 2, 0.921 4 and 0.933 9, respectively. In the meantime all the data could be well non-linear fitted by Weibull equation（in full concentration range）. Their correlation coefficients R2 were 0.974 9, 0.974 2 and 0.964 8 respectively. The results were satisfied and their EC50 values were 1.49E-04, 2.72E-04 and 9.00E-06 mol·L^-1. The both fitted data expressed that the Weibull non-linear was more fitted and their EC50 values were similar. By the results, for DMP, DEP and DBP with respective concentration ranges of 6.14E-9-6.14E-3, 5.04E-5-2.52E-3 and 3.76E-9-3.76E-5 mol·L^-1, the acute toxicity to Photobacterium phosphoreum T3 was DBP DMP DEP. In the concentration ranges, the inhibiton ratios for DMP

关 键 词：酞酸酯(PAEs) DMP DEP DBP 明亮发光杆菌(Photobacterium phosphoreum) 

分 类 号：X503[环境科学与工程—环境工程]