姜黄素抑制人宫颈癌CaSki细胞裸鼠移植瘤的生长  

Inhibitory effect of curcumin on the growth of human cervical carcinoma( CaSki) xenografts in nude mice

作　　者：刘冬菊[1] 姚宇[1] 黄文革[2] 

机构地区：[1]广州中医药大学第三附属医院,广州510360 [2]中山大学实验动物中心,广州510080

出　　处：《中国比较医学杂志》2015年第2期7-10,I0001,共5页Chinese Journal of Comparative Medicine

基　　金：广东省科技计划基金项目(编号:2012B061700029)

摘　　要：目的探讨姜黄素对人宫颈癌Ca Ski细胞裸鼠皮下移植瘤生长的抑制作用。方法建立人宫颈癌裸鼠皮下移植瘤模型,35只荷瘤裸鼠随机分为5组,姜黄素高剂量(200 mg/kg)、中剂量(100 mg/kg)、低剂量(50mg/kg)组、溶剂对照组和顺铂组(3 mg/kg),每组7只,姜黄素连续灌胃给药15 d,顺铂组每3 d腹腔注射给药,共5次。停药24 h后处死裸鼠,测量裸鼠体重、移植瘤体积、瘤重,计算抑瘤率;光镜下观察移植瘤组织形态学变化;结果处理组的移植瘤体积均明显小于空白对照组(P<O.01)。姜黄素高、中、低剂量组抑瘤率分别为44.3%、40.1%和33.3%;HE染色法显示姜黄素处理组小鼠的移植瘤组织出现明显坏死改变;结论姜黄素对人宫颈癌Ca Ski细胞移植瘤的生长具有明显的抑制作用。Objective To study the inhibitory effect of curcumin on the growth of human cervical carcinoma Ca Ski xenografts in nude mice. Methods Human cervical cancer cell line Ca Ski cells were inoculated subcutaneously into nude mice. Thirty-five tumor-bearing mice were randomly divided into 5 groups： curcumin high dose（ 200 mg / kg）,moderate dose（ 100 mg / kg）,low dose（ 50 mg / kg）,solvent control group and cisplatin group（ 3 mg / kg）,7 mice in each group. Curcumin was orally administered once per day for consecutive 15 days. Intraperitoneal injection of cisplatin was given every three days,for a total of five times. The mice were sacrificed 24 h after the end of treatment and the body weight,tumor volume,tumor weight were measured and the inhibition rate was calculated. The histological changes of tumor tissue was examined. Results The tumor volumes of the treated group were significantly lower than that of the control group（ P〈0. 01）. The tumor inhibition rate of the curcumin high,medium and low dose groups were 44. 3%,40. 1% and 33. 3%, respectively. Apparent necrosis was observed in the tumors of the curcumin-treated groups.Conclusion Curcumin possesses apparent inhibitory effect on the growth of transplanted tumors of human cervical cancer Ca Ski cells in nude mice.

关 键 词：宫颈癌 姜黄素 移植瘤 

分 类 号：R33[医药卫生—人体生理学]