枸杞对小鼠辐射损伤后造血系统修复的促进作用  

Promoting effect of Lycium chinense Mill on hematopoietic reconstruction in mice after radiation injury

作　　者：石桂英[1] 白琳[1] 

机构地区：[1]中国医学科学院北京协和医学院实验动物研究所,卫生部人类疾病比较医学重点实验室,北京100021

出　　处：《中国比较医学杂志》2015年第2期38-41,66,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金青年科学基金项目[No.81200256];北京市自然基金项目[No:7132161]

摘　　要：目的探讨枸杞在小鼠辐射损伤后造血系统修复过程中的作用。方法小鼠随机分为3组,正常对照组不做任何处理,喂饲正常饲料;另外2组,给予4.5 Gy照射后,喂饲正常饲料或添加枸杞的饲料,1个月后,处死小鼠,应用流式细胞仪分析外周血、脾脏、胸腺及骨髓中各种血细胞。结果枸杞饲料组小鼠,外周血细胞总数及B细胞增多,骨髓细胞总数减少,骨髓前体B细胞及未成熟B细胞增多,造血干、祖细胞均显著减少。结论枸杞促进辐射损伤后造血干细胞动员及分化,加速造血系统的恢复。Objective To investigate the effect of Lycium chinense Mill on hematopoietic reconstruction in mice after radiation injury. Method Female C57 BL /6J mice were grouped to 3 groups at random： the control group,with normal food and no treatment,and the other 2 groups,treated with 4. 5 Gy irradiation,with normal food or food added with Lycium chinense Mill. After 1 month,the mice were sacrificed and analyzed the peripheral blood,spleen,thymus and bone marrow cells by flow cytometer. Results In the mice fed with Lycium chinense Mill,the percentage of B cells was increased in the peripheral blood,the pre-B and immature B cells were increased,too,and the percentage of hematopoietic stem / progenitor cells was decreased. Conclusion Lycium chinense Mill can promote the mobilization and differentiation of hematopoietic stem / progenitor cells,and accelerate the hematopoietic reconstruction after irradiation injury.

关 键 词：枸杞 辐射损伤 流式细胞术 造血干祖细胞 

分 类 号：R33[医药卫生—人体生理学]