巨细胞病毒感染新生小鼠辅助性T细胞1/2类细胞因子及其转录因子表达变化及胸腺五肽的干预作用  

Changes in help T cell 1/2 cytokines and transcription factors in cytomegalovirus infected newborn mice and effectiveness of intervention with thymopentin

作　　者：叶黎离[1] 王军[1] 

机构地区：[1]徐州医学院附属医院儿科,221002

出　　处：《中华围产医学杂志》2015年第2期117-122,共6页Chinese Journal of Perinatal Medicine

基　　金：江苏省“六大人才高峰计划”基金项目（2011-WS075）

摘　　要：目的 观察巨细胞病毒（cytomegalovirus,CMV）感染新生小鼠辅助性T细胞（help T cell,Th）1/2类细胞因子及其转录因子表达水平变化,并探讨胸腺五肽对其产生的干预作用. 方法 120只新生BALB/c小鼠随机分为对照组、模型组和胸腺五肽组（每组40只）.对照组仅腹腔注射等容积生理盐水;模型组和胸腺五肽组于生后4～6 h内腹腔注射组织半数细胞感染量（tissue culture infections dose,TCID50）为10 4.31U/0.1 ml的鼠巨细胞病毒（murine cytomegalovirus,MCMV）病毒悬液20μl,建立全身播散性感染MCMV模型.胸腺五肽组腹腔注射胸腺五肽0.3 mg/kg,每天1次.生后第3、5、7、10和14天无菌分离脾组织,酶联免疫吸附试验法检测脾淋巴细胞培养上清液中的干扰素（interferon,IFN）-γ和白细胞介素（interleukin,IL）-10表达;逆转录-聚合酶链反应技术检测脾组织转录因子T-bet（T-box expressed in T cells,T-bet）mRNA和转录因子Gata3（Gata-binding protein-3,GATA-3）mRNA表达.采用单因素方差分析（包括LSD法和Dunnett T3法）进行统计学分析. 结果 （1）IFN-γ表达：模型组和胸腺五肽组于第3天达高峰,均明显高于对照组[分别为（280.73±14.88）、（286.03±15.44）和（149.42±5.43） pg/ml,F=183.532,P=0.000].胸腺五肽组第3天后迅速降低,第7天起回升,至第14天高于对照组和模型组[分别为（252.33±8.33）、（149.07±7.05）和（148.57±4.53）pg/ml,F=385.487,P=0.000].（2）IL-10表达：模型组感染MCMV后IL-10表达水平逐渐升高,第14天明显高于对照组和胸腺五肽组[分别为（71.19±1.50）、（36.67±2.55）和（40.01±1.28） pg/ml,F=523.670,P=0.000];胸腺血肽组IL-10表达水平先升高,第7天后下降,至第14天明显低于模型组,但仍高于对照组.（3） T-bet mRNA表达：模型组和胸腺五肽组感染后T-bet mRNA表达迅速增强,第3天达高峰,均明显高于对照组（相对吸光度值分别为0.74±0.02、0.71±0.04和0.30±0.01,FObjective To observe the changes in help T cell 1/2 cytokines and transcription factors in murine cytomegalovirus （MCMV） infected newborn mice and to determine the effectiveness of intervention with thymopentin.Methods One hundred and twenty newborn BALB/c mice were randomized into a blank group,model group and thymopentin group,with 40 mice in each group.Mice in the model and thymopentin group received an intraperitoneal injection of MCMV suspension within 4-6 hours after birth at the dosage of 20 μ L to and tissue culture infections dose of 10431 U/0.1 ml,establish a systemic infection model,and the same volume of normal saline was injected into mice in the control group.Mice in the thymopentin group also received thymopentin 0.3 mg/（kg · d）.On Day 3,5,7,10 and 14,8 mice in each group were sacrificed and their splenic tissues were harvested.Interferon （IFN） γ and interleukin （IL）-10 expression in the supernatant of a splenic lymphocyte culture was assessed by enzyme-linked immunosorbent assay.T-bet/GATA-3 mRNA in splenic tissues were analyzed using reverse transcription-polymerase chain reaction.One way ANOVA,including LSD and Dunnett T3 methods,was used for statistical analysis.Results （1） IFN-γ expression in the model and thymopentin groups peaked on Day 3,and was higher than that in the control group [（280.73 ± 14.88）,（286.03 ± 15.44） and （149.42±5.43） pg/ml,respectively,F=183.532,P=0.000].Expression in the thymopentin group decreased after Day 3,and increased by Day 7.At Day 14,the IFN-γ expression level in the thymopentin group was higher than that in the control and model groups [（252.33±8.33）,（149.07±7.05） and （148.57±4.53） pg/ml,respectively,F=385.487,P=0.000].（2） IL-10 expression in the model group gradually increased.By Day 14,the expression became obviously higher than that in the control and thymopentin groups [（71.19± 1.50）,（36.67 ± 2.55） and （40.01 ± 1.28） pg/ml,respectively,F=523.670,P=0.000].IL-10 expression in the thymope

关 键 词：巨细胞病毒感染 胸腺喷丁 干扰素Γ 白细胞介素10 转录因子 GATA3转录因子 

分 类 号：R722.1[医药卫生—儿科]