Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas(Thunberg)  

作　　者：王宜艳 孙虎山 王艳杰 闫冬春 王磊 

机构地区：[1]College of Life Science,Ludong University

出　　处：《Chinese Journal of Oceanology and Limnology》2015年第2期339-346,共8页中国海洋湖沼学报（英文版）

基　　金：Supported by the Natural Science Foundation of Shandong Province(Nos.ZR2010CM017,ZR2012CM004);the National Natural Science Foundation of China(No.31101929)

摘　　要：In this study, a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosphatase, and the mechanisms underlying its involvement in yolk degradation during the early developmental stages of molluscs. Three types of yolk granules （YGI, YGⅡ, and YGⅢ） that differed in electron density and acid phosphatase reaction were identified in early cleavage, morula, blastula, gastrula, trochophore, and veliger stages. The morphological heterogeneities of the yolk granules were related to acid phosphatase activity and degrees of yolk degradation, indicating the association of acid phosphatase with yolk degradation in embryos and larvae of molluscs. Fusion of yolk granules was observed during embryogenesis and larval development of C. gigas. The fusion of YGI （free of acid phosphatase reaction） with YGII （rich in acid phosphatase reaction） could be the way by which yolk degradation is triggered.In this study,a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosphatase,and the mechanisms underlying its involvement in yolk degradation during the early developmental stages of molluscs. Three types of yolk granules(YGI,YGII,and YGIII) that differed in electron density and acid phosphatase reaction were identified in early cleavage,morula,blastula,gastrula,trochophore,and veliger stages. The morphological heterogeneities of the yolk granules were related to acid phosphatase activity and degrees of yolk degradation,indicating the association of acid phosphatase with yolk degradation in embryos and larvae of molluscs. Fusion of yolk granules was observed during embryogenesis and larval development of C. gigas. The fusion of YGI(free of acid phosphatase reaction) with YGII(rich in acid phosphatase reaction) could be the way by which yolk degradation is triggered.

关 键 词：CYTOCHEMISTRY EGGS embryo larvae molluscs Pacific oyster yolk granules 

分 类 号：S917.4[农业科学—水产科学]