RP-HPLC测定地佐辛的含量  

Content Determination of Dezocine by RP-HPLC

作　　者：祖若玉[1] 徐凌霞[1] 杨敏[1] 管晓琴[1] 蔡亚兰[1] 李燕[1] 

机构地区：[1]扬子江药业集团质量管理部,江苏泰州225321

出　　处：《中国执业药师》2015年第2期28-30,共3页China Licensed Pharmacist

摘　　要：目的：建立地佐辛含量测定的反相高效液相色谱法（RP-HPLC）。方法：色谱柱为SHIMADZV VP-ODS（250 mm×4.6 mm;5μm）,流动相为乙腈-三乙胺溶液（20∶80）,检测波长：281 nm,流速：1.0 m L/min,柱温：30℃,进样量：20μL。结果：地佐辛在0.05~0.30 mg/m L范围内线性关系良好,r=1.000 0;平均回收率为100.2%（RSD=1.0%）。结论：该法操作简便、结果准确,可以用于地佐辛的定量分析。Objective： To establish a method for the content determination of dezocine by reversed phase high-performance liquid chromatography （RP-HPLC） method. Methods ： SHIMADZV VP-ODS （250 mm×4.6 mm;5μm） column was used with the mobile phase of acetonitrile three triethylamine solution （20：80）, the detection wavelength was at 281 nm, the flow rate was 1 mL/min, the column temperature was at 30 ℃, and the sample size was 20 μL. Results： A good linear relationship of dezocine was within the range of 0.05 ~ 0.30 mg/mL （r = 1.000 0）,the average recovery rate was 100.2% （RSD = 1.0%）. Conclusion： The method is simple and accurate, and can be used for quantitative analysis of dezocine.

关 键 词：反相高效液相色谱法 地佐辛 含量测定 

分 类 号：R286.0[医药卫生—中药学]