壮骨止痛方对去势雌鼠ERRα基因表达及血清E_2水平的影响  

Effects of Zhuanggu Zhitong prescription on gene expression of estrogen- related receptor α and serum E_2 level in ovariectomized rats

作　　者：王文杰[1] 曾英[1] 李劲平[2] 张国民[1] 刘珊[1] 杨琳[1] 杨岩冰[1] 莫新民[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]中南大学药学院,湖南长沙410013

出　　处：《湖南中医杂志》2015年第2期122-124,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81273816)

摘　　要：目的:从雌激素/雌激素相关受体信号通路探讨壮骨止痛方抗绝经后骨质疏松症的作用机制。方法:将60只雌性SD大鼠建立去卵巢骨质疏松症病理模型,将其随机分为壮骨止痛方高、中、低剂量组,模型组,雌二醇组,每组12只;另将12只雌性SD大鼠只切除卵巢附近等体积的脂肪组织作为假手术组。各组分别采用相对应药物进行灌胃治疗,治疗13周后各组随机选取6只雌鼠提取股骨组织mRNA样本进行PCR反应检测ERRα基因的表达,选取8只雌鼠经腹主动脉采血,凝固后离心分离出血清,应用雌二醇(E2)ELISA试剂盒检测血清E2水平。结果:与假手术组比较,模型组E2浓度及骨组织ERRα基因表达均显著降低,差异有统计学意义(P<0.01);与模型组比较,壮骨止痛方高、中剂量组及阳性对照组血清E2浓度及骨组织ERRα基因表达均显著升高,差异有统计学意义(P<0.01,P<0.05),壮骨止痛方低剂量组较模型组升高,但差异无统计学意义(P>0.05)。结论:壮骨止痛方通过同时提高去势雌鼠血清E2水平和骨组织ERRαmRNA表达来发挥抗绝经后骨质疏松症的作用。Objective： To investigate the action mechanism of Zhuanggu Zhitong prescription in the treatment of postmenopausal osteoporosis from the perspective of estrogen / estrogen- related receptor signaling pathway. Methods：Sixty female Sprague- Dawley（ SD） rats were ovariectomized to establish a rat model of osteoporosis. And then they were equally and randomly divided into high- dose,middle- dose,and low- dose Zhuanggu Zhitong prescription groups,model group,and estradiol group（ n = 12 for each）. Another 12 female SD rats,from which only the same volume of adipose tissue near the ovary was resected,were used as sham- operation group. The Zhuanggu Zhitong prescription groups and estradiol group were given Zhuanggu Zhitong prescription and estradiol,respectively,by gavage; the model group was given saline by gavage; the sham- operation group was fed conventionally. After 13 weeks of treatment,the mRNA samples extracted from the femoral tissues of 6 rats,which were randomly selected from each group,were subjected to PCR to measure the gene expression of estrogen- related receptor α（ ERRα）.The blood samples were taken from the abdominal aorta of 8 rats,which were randomly selected from each group,and the sera separated from the clotted blood samples by centrifuging were used to measure estrogen 2（ E2） level with the estradiol enzyme- linked immunosorbent assay kit. Results： The model group had significantly reduced E2 level and gene expression of ERRα than the sham- operation group（ P〈0. 01）. Compared with the model group,the high- dose and middle- dose Zhuanggu Zhitong prescription groups and the estradiol group had significantly increased E2 level and gene expression of ERRα（ P〈0. 01 or P〈0. 05）,while these parameters were nonsignificantly increased in the low- dose Zhuanggu Zhitong prescription group（ P〉0. 05）. Conclusion： Zhuanggu Zhitong prescription plays the role of treating postmenopausal osteoporosis by increasing the serum E2 level and ERRα mRNA expression in ovar

关 键 词：绝经后骨质疏松症 大鼠 壮骨止痛方 雌激素相关受体α 雌二醇 实验研究 

分 类 号：R285.5[医药卫生—中药学]