蓬子菜总黄酮对急性血瘀大鼠血液流变学影响及作用机制的研究  

Ameliorative Effect on Hemorheology and Mechanism of Galium Verum L Flavonoids in Rats with Acute Blood Stasis

作　　者：寇韩旭 董坤[1] 宁馨[1] 孙超[1] 马英丽[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2015年第1期11-15,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81274034);教育部博士点博导类基金资助项目(20112327110006)

摘　　要：目的:研究蓬子菜总黄酮对急性血瘀大鼠血液流变学、凝血功能的影响,及对炎症、氧化损伤和血管舒缩功能的影响,确定药物的治疗作用及治疗机制。方法:采用皮下注射盐酸肾上腺素加冰水浴刺激的方法制备大鼠急性血瘀模型;下腔静脉采血,检测血液流变学指标、凝血四项指标、氧化损伤指标、血管调控物质及炎症因子。结果:蓬子菜总黄酮可明显改善血流变各项指标,明显降低血浆FIB、MDA、CRP、TNF-α、IL-1β、s ICAM-1含量,明显延长PT、APTT、TT时间,明显增加血清NO及血浆SOD含量(P<0.01或P<0.05)。结论:实验结果表明,蓬子菜总黄酮能够明显改善急性血瘀大鼠血流变学相关指标,抗凝血、抗氧化损伤、抗炎、调节血管舒缩功能方面作用明显;其中黄酮中剂量组作用效果较好。Objective： Through the experiment to study the effects of flavonoids which were extracted from Galium verum L on the changes of hemorheology and the effect of total flavonoids＇ antioxidant and anti- inflammation on acute blood stasis model rats,in order to determine the therapeutic effect and mechanism of drug treatment. Methods： The models of acute blood stasis rats were built by subcutaneous injection of adrenaline hydrochloride and ice water bath. Draw the rats＇ blood from the inferior vena cava to test the changes of hemorheology and blood constituents. Results： Flavonoids of Galium verum L flavonoids could significantly reduce blood viscosity of acute blood stasis rats and could significantly reduce the contents of FIB,MDA,CRP,TNF- α,IL- 1 β and s ICAM- 1 in blood. It could also significantly prolong the time of PT,APTT and TT,and could significantly increase the contents of SOD and NO in blood（ P〈0. 01 or P〈0. 05）. Conclusion： The results show that total flavonoids of Galium verum L can significantly improve the model group rats＇ blood hemorheology index,and they have antioxidant and anti- inflammatory effect. They can also significantly regulate vasomotor function. In addition,the effect of medium dose of flavonoid group is superior to the positive drug group.

关 键 词：蓬子菜总黄酮 急性血瘀 血液流变学 

分 类 号：R285.5[医药卫生—中药学]