大鼠脑挫伤后caspase-3和HAX-1的表达  

Expression of caspase-3 and HAX-1 after Cerebral Contusion in Rat

作　　者：李周儒[1] 滕道辉 董国凯[1] 殷文江[1] 蔡红星[1] 

机构地区：[1]徐州医学院法医学教研室,江苏徐州221002 [2]徐州市铜山区公安局刑警大队,江苏徐州221100

出　　处：《法医学杂志》2015年第1期7-10,14,共5页Journal of Forensic Medicine

基　　金：江苏省"六大人才高峰"第七批次项目(032)

摘　　要：目的观察大鼠脑挫伤后半胱氨酸天冬氨酸蛋白酶3(caspase-3)和造血干细胞特异性相关结合蛋白-1(HCLS1-associated protein X-1,HAX-1)在损伤后不同时段的表达规律,探索损伤时间推断的新方法。方法建立成年SD雄性大鼠脑挫伤模型,随机分为脑挫伤后2 h、6 h、12 h、1 d、3 d、7 d组,假手术组和正常组。采用Western印迹法检测大鼠脑挫伤后caspase-3和HAX-1的蛋白表达。采用激光共聚焦显微镜检测技术对脑挫伤后HAX-1阳性细胞数和TUNEL染色细胞数进行观察。结果脑挫伤后,随着损伤时间的延长,caspase-3表达逐渐增强,3 d达到峰值,以后逐渐下降,7 d仍高水平表达(P<0.05)。HAX-1阳性细胞表达在伤后开始升高,6 h表达强度最高(P<0.05),12 h后逐渐下降,伤后3 d几乎测不到。TUNEL染色细胞数在伤后2 h明显增加,伤后3 d数量最多,此后逐渐减少,7 d仍维持较高水平(P<0.05)。结论大鼠脑挫伤后caspase-3、HAX-1的表达有时序性变化,可能成为脑损伤时间推断的新依据。Objective To observe the expression pattern of caspase-3 and HCLS1-associated protein X-1 （HAX-1） at different time after cerebral contusion in rat, and explore the new method for estimating the injury interval. Methods The cerebral contusion model was established using adult SD male rats. Then the rats were randomly allocated into 8 groups： 2 h, 6 h, 12 h, 1 d, 3 d, and 7 d after cerebral con-tusion, sham-operation and normal control. Expression of caspase-3 and HAX-1 protein after cerebral contusion in rat was detected by Western blotting. Laser scanning confocal microscope was used to ob-serve the number of HAX-1 positive cells and TUNEL-stained cells after cerebral contusion. Results The expression of caspase-3 increased parallelly with the time after cerebral contusion and reached the peak value on 3 d. The expression of caspase-3 decreased gradually and still maintained a high level expression on 7 d （P〈0.05）. The expression of HAX-1 positive cell went up after injury, and reached the peak value at 6 h （P〈0.05）, then turned down gradually after 12 h and went out of detection after 3 d. The number of TUNEL-stained cells increased obviously at 2 h and reached the peak value on 3 d. The number of TUNEL-stained apoptotic cells decreased gradually and still maintained a high level expres-sion on 7 d （P〈0.05）. Conclusion The expression of caspase-3 and HAX-1 after cerebral contusion has time sequential regularity, which may provide new evidence for forensic diagnosis of cerebral contusion interval.

关 键 词：法医病理学 脑损伤 半胱氨酸天冬氨酸蛋白酶3 造血干细胞特异性相关结合蛋白-1 大鼠 

分 类 号：DF795.1[医药卫生—法医学]