皮肤扩张急性期miRNA表达谱的变化及关键miRNA的筛选  

mi RNA Expression and Key mi RNA Screening in Acute Stage of Skin Expansion

作　　者：刘文辉[1] 黄晓璐[1] 李海洲[1] 余庆雄 梁筱[1] 周怡雯[1] 李青峰[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海市200011

出　　处：《组织工程与重建外科杂志》2015年第1期10-13,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(No:81230042)

摘　　要：目的明确扩张急性期miRNA表达谱的变化,筛选出其中的关键miRNA。方法建立大鼠皮肤扩张模型,在扩张急性期取材,以未扩张皮肤为对照,利用miRNA芯片检测miRNA表达谱的变化,并利用t检验和差异表达的倍数变化,筛选出关键miRNA。利用TargetScan和miRDB数据库预测关键miRNA的靶点,并通过KEEG和IPA数据库对预测靶点进行通路分析,以探究关键miRNA调控皮肤扩张急性期的可能机制。结果本研究筛选出11个关键miRNA,这些关键miRNA调控多个与增殖分化、血管化、纤维化以及肿瘤发生发展相关的通路。结论本研究筛选出皮肤扩张急性期的11个关键miRNA,调控扩张过程中多个重要通路的变化,可以作为调控皮肤扩张的靶点。Objective To determine the miRNA expression and screen key miRNA in acute stage of skin expansion.Methods Skin expansion model was established and skin samples were harvested in acute stage. Unexpanded skin was also harvested as control. miRNA microarray was undertaken to reveal the miRNA expression change during acute expansion process. T test and fold change of differential expression were applied to screen key miRNAs. Target Scan and mi RDB were used to predict target genes of key miRNA. Pathway analysis was performed for target genes by KEEG and IPA. Results Eleven key miRNAs were detected which regulate pathways involve cell proliferation, differentiation, vascularization, fibrosis and tumorigenesis. Conclusion Eleven key miRNAs determined in the present study regulate important pathways in acute stage of skin expansion and they could serve as targets in skin expansion.

关 键 词：皮肤扩张 小分子核糖核酸 通路分析 

分 类 号：R622[医药卫生—整形外科]