磷霉素联合左氧氟沙星对鲍曼不动杆菌生物膜的体外研究  

Effect of fosfomycin combined with levofloxacin on Acinetobacter baumannii biofilm

作　　者：晏奎[1] 温汉春[1] 陈一强[2] 梁宏洁[3] 李萌[3] 闵利[1] 

机构地区：[1]广西医科大学第一附属医院急诊科,南宁530021 [2]广西医科大学第一附属医院呼吸科,南宁530021 [3]广西医科大学第一附属医院医学检验科,南宁530021

出　　处：《中国临床药理学杂志》2015年第4期276-278,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81260663);广西教育厅基金资助项目(200710MS156)

摘　　要：目的探讨磷霉素(FOS)对鲍曼不动杆菌生物膜的破坏作用以及与左氧氟沙星(LFX)的联合杀菌效果。方法选取临床分离鲍曼不动杆菌菌株构建体外生物膜模型,微量肉汤稀释法测定FOS及LFX的最低抑菌浓度(MIC),连续稀释法测定生物膜内活菌计数,结晶紫染色法半定量生物膜。用单因素方差分析进行统计处理。结果生物膜经抗生素作用24 h后,生物膜半定量显示,FOS组及FOS+LFX组吸光度值均少于空白对照组,差异有统计学意义(P<0.01);但LFX组吸光度值与空白对照组比较,差异无统计学意义。FOS组及LFX组的生物膜内活菌计数与空白对照组比较,差异无统计学意义;FOS+LFX组第4,8,24 h膜内活菌计数均少于空白对照组(P<0.01)。结论 FOS能破坏鲍曼不动杆菌已形成的生物膜,并可增强LFX对生物膜内鲍曼不动杆菌的清除作用。Objective To observe the in vitro destructive effect of fosfomycin（ FOS） on the biofilm of Acinetobacter baumannii and the synergistic antibacterial activity in combination with levofloxacin（ LFX）. Methods The biofilm model was established by clinical isolates of Acinetobacter baumannii- 48876 as the study strain. The minimum inhibitory concentrations（ MIC） of FOS and LFX were measured by doubling dilution. The viable count of biofilm carrier were determined by continuous dilution method and the biofilm quantitation by crystal violet staining method. Data were analyzed by single factor analysis of variance（ One- Way ANVOA）. Results When the biofilm being reacted with antibiotic for 24 hours,the quantitation of biofilm showed the absorbances of FOS group and FOS plus LFX group were significantly less than that of the control group（ P〈0. 01）. While there was no statistical difference found in absorbance value in LFX group and the control group. At the 4th,8thand24 thhour,the number of bacteria in biofilm of FOS plus LFX group was significantly less than that in the control group（ P〈0. 01）,While there was no statistical difference found between FOS or LFX group and control group. Conclusion FOS can destroy the biofilm formed by Acinetobacter baumannii,and the Acinetobacter baumannii in the biofilm carrier could be significantly reduced while plus LFX.

关 键 词：鲍曼不动杆菌 生物膜 磷霉素 左氧氟沙星 杀菌作用 

分 类 号：R978.1[医药卫生—药品] R969.3[医药卫生—药学]