离子液体作为功能单体制备溶菌酶分子印迹复合膜的研究  

Lysozyme Molecular Imprinted Membranes with Ionic Liquids as Functional Monomer

作　　者：王超丽[1] 胡小玲[1] 管萍[1] 吴丹锋 钱立伟[1] 李季[1] 宋任远[1] 

机构地区：[1]西北工业大学理学院教育部空间应用物理与化学重点实验室,西安710072

出　　处：《高分子学报》2015年第3期259-265,共7页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号21174111)资助项目

摘　　要：以1-乙烯基-3-乙酸乙酯咪唑氯离子液体为功能单体,以再生纤维素膜为基膜,采用温和的表面ATRP接枝聚合技术在水溶液中制备了溶菌酶分子印迹膜.通过紫外-可见光谱分析了离子液体与模板分子的作用力,讨论了功能单体1-乙烯基-3-乙酸乙酯咪唑氯用量对印迹复合膜性能的影响,研究了分子印迹复合膜对溶菌酶模板分子及其结构类似物的吸附行为和选择性识别特性.结果表明,以1-乙烯基-3-乙酸乙酯咪唑氯离子液体作为功能单体的溶菌酶分子印迹膜能够从结构类似物混合体系中选择性分离富集溶菌酶,且具有很好的稳定性和可再生性能.In order to increase structural selectivity,surface-initiated ATRP and ionic liquid（ 1-vinyl-3-ethyl acetate imidazolium chloride） as functional monomer were used to prepare molecularly imprinting membranes（ MIMs）. MIMs were prepared with lysozyme as template,ionic liquids（ 1-vinyl-3-ethyl acetate imidazolium chloride） as functional monomer,MBA as cross-linking agent,PMDETA as ligand,Cu Cl as catalyst through surface-initiated ATRP on the initiator-functional regenerated cellulose membranes in aqueous solution at 35℃. The imprinted density and thickness of lysozyme molecular imprinted membranes were controlled by varying the concentrations of functional monomer. The imprinting factor α reaches the maximum（ 6. 25） at the ratio of functional monomer to initiator 100∶ 1,with the water flux 83. 0 L / m^2/ h. The equilibrium experimental data for the lysozyme-MIMs fitted the Langmuir isotherm well,and the binding amount reached 14. 8 mg / g. The solid phase extraction experiments demonstrated that lysozyme-MIMs had specific separation and recognition performance in 4 min. Furthermore,permeability analysis experiments demonstrated that the lysozyme-MIMs had higher penetration ability for the target molecules than competitive molecules Cyt C and BHb. Based on the data obtained in separation of lysozyme from the mixed solution,the lysozyme-MIMs separation mechanism belongs to facilitated permeation,the template molecular lysozyme is first specifically adsorbed and concentrated,and then moves through the imprinted membrane by the binding / desorption process.

关 键 词：离子液体 分子印迹 ATRP 再生纤维素膜 溶菌酶 

分 类 号：O631.3[理学—高分子化学]