聚砜共价键合磷铵两性离子的结构修饰及其血液相容性的研究  

Polysulfones with Covalently-attached Zwitterionic Phosphorylcholine Structure for Improving Blood Compatibility

作　　者：金灿[1] 江玉亮[2] 韩巧荣[2] 沈健[2] 王炳祥[2] 

机构地区：[1]中国林业科学院林产化学工业研究所生物质化学利用国家工程实验室江苏省生物质能源与材料重点实验室,南京210042 [2]江苏省生物功能材料重点实验室江苏省生物医药功能材料工程研究中心南京师范大学化学与材料科学学院,南京210097

出　　处：《高分子学报》2015年第3期306-311,共6页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号20874047)资助项目

摘　　要：利用形成碳-氧键将磷铵两性离子(1)共价键合到聚砜(PSF)材料表面,改善其抗凝血性能.首先对聚砜(PSF)进行氯甲基化反应,生成苄氯结构,然后通过自合成两性离子化合物1中的—OH与氯甲基化聚砜的—CH2Cl反应形成醚键,将两性离子结构接枝在PSF上.用ATR-FTIR、EA和1H-NMR表征了产物结构,并通过水接触角、溶血实验和血小板黏附实验对结构修饰前后材料的亲水性和抗凝血性能进行了比较.改性PSF材料的表面亲水性提高,几种改性PSF材料的溶血率均低于5%,PSF-18.1%1材料的表面几乎没有血小板黏附.结果表明,磷铵两性离子结构修饰的聚砜材料可以显著提高其血液相容性,在血液相容性材料等领域具有潜在的应用价值.In order to improve the hemocompatibility polysulfones（ PSF） covalently-attached compound 1（ HTEP） containing phosphorylcholine structure via ether bond were prepared. Firstly,PSF was activated with chloromethylation reagent to give chloromethylated polysulfone 2（ CMPSF）. Then compound 2 reacted with compound 1 by the reaction between benzyl chloride and hydroxyl group to give phosphorylcholine-coated PSF（ PSF-HTEP）（ 3）. The grafted materials 3 with different grafting ratios of compound 1 were characterized by attenuated total reflection Fourier transform infrared spectroscopy（ ATR-FTIR）,element analysis（ EA） and H-atom nuclear magnetic response spectroscopy（1H-NMR）. The surface properties of PSF-HTEP（ 3）membranes were tested by contact angle measurements and the results showed that the membranes of grafted materials 3 possessed more hydrophilic surface with the increase of the number of grafting ratio of compound 1,as expected. The blood compatibilities of membranes（ 3） were evaluated by testing their hemolytic properties and comparing the experimental results of platelet-rich plasma adhesion of PSF-HTEP（ 3）. The results showed that all hemolytic rates of membranes（ 3） with different grafted ratios were less than 5% and that the number of platelet adhesion on the surface of PSF-HTEP（ 3） decreased dramatically with the increase of graft ratio of1,and in particular even no platelet adhesion was observed on the surface of PSF-18. 1% 1 membrane. Such a novel PSF-HTEP（ 3） containing phosphorylcholine structure showed improving blood compatibility,which was supported by the hypothesis of ＂maintaining natural behavior ＂ and revealed a promising potential use in hemocompatibility materials.

关 键 词：聚砜 磷铵两性离子 表面改性 血小板黏附 抗凝血生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]