靶向MPO的磁性纳米粒子的制备与表征  

Synthesis and Characterization of Magnetic Nanoparticles Targeting for MPO

作　　者：刘环[1] 赵外欧[2] 黄海龙[1] 胡齐[1] 李亚鹏[1] 陈亮[2] 王静媛[1] 

机构地区：[1]吉林大学化学化学学院麦克德尔米德实验室,长春130012 [2]吉林大学第一医院,长春130012

出　　处：《高分子学报》2015年第3期331-337,共7页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号20904014);吉林省科技厅青年基金(基金号20080119);教育部青年基金(基金号2070310)资助项目

摘　　要：将表面含有大量氨基的树枝状分子聚酰胺-胺与油溶性的氧化铁纳米粒子(IONPs)进行配体交换,制备了水溶性氧化铁纳米粒子,将动脉粥样硬化斑块中髓过氧化物酶(MPO)的靶向分子5-羟色胺(5-HT)接枝到以树枝状分子为载体的体系上,最后得到树枝状分子包覆IONPs纳米粒子-g-PEG-g-5-羟色胺(5-HTPEG-G3.0@IONPs),可以作为一种靶向动脉粥样硬化斑块的核磁造影成像剂.通过测试产物的红外光谱(FTIR),证明合成了5-HT-PEG-G3.0@IONPs纳米粒子.TEM测试结果表明该纳米粒子的的粒径约为10 nm左右,DLS测试结果显示的粒径约为31.0 nm.利用5-HT具有紫外特征吸收峰(276 nm),确定了终产物中5-HT的接枝量为6.19μg/mg.TGA结果表明5-HT-PEG-G3.0@IONPs的氧化铁含量为2 wt%.VSM测试结果表明该产物具有超顺磁性,且饱和磁化强度为1.47 A·m2/kg.In order to form the hydrophilic nanoparticles,the oleylamine ligand of oil-soluble iron oxide nanoparticles（ IONPs） were ligand-exchanged with the amine groups of poly（ amidoamine） dendrimers. Then the targeting molecules 5-hydroxytryptamine（ 5-HT） which can target myeloperoxidase in the atherosclerotic plaque were grafted on the dendritic polymer coating of nanoparticles. Finally,a targeted contrast imaging system（ poly（ amidoamine） dendrimers coat IONPs-graft-PEG-graft-5-HT,5-HT-PEG-G3. 0 @ IONPs） which can target to atherosclerotic plaque and image for magnetic resonance imaging was achieved. Structure of all the samples were characterized by FTIR which could confirm that the products were successfully prepared. The sizes of particles were tested by dynamic light scattering（ DLS） and also observed under transmission electron microscope（ TEM）. According to the tests,the nanoparticles showed a spherical and monodispersed structure,and DLS result showed that the diameters of 5-HT-PEG-G3. 0@ IONPs were about 31 nm and the TEM result was about 10 nm,respectively. The standard curve of 5-HT was portrayed based on the characteristic absorption peak at 276 nm in the UV-Vis test. Then the amount of 5-HT grafted on the final products was defined and the content of 5-HT was 6. 19 μg / mg. The results of thermogravimetric analysis（ TGA） showed that about 2 wt%iron oxide was in the nanoparticles,and vibrating sample magnetometer（ VSM） revealed the superparamagnetism of 5-HT-PEG-G3. 0@ IONPs nanoparticles,and the saturation magnetization was 1. 47 A·m2/ kg.

关 键 词：磁性氧化铁纳米粒子 髓过氧化物酶 5-羟色胺 靶向核磁造影剂 

分 类 号：TQ421.7[化学工程] TB383.1[一般工业技术—材料科学与工程]