HPLC法测定复方苦参败酱草保留灌肠剂中齐墩果酸含量  

Determination of Oleanic Acid in Compound Sophora Herba Patriniae Retention Enema by HPLC

作　　者：李成[1] 路伟[1] 巫小娟 蔡柳清 曾繁涛[1] 卓丽婷 颜晗[3] 

机构地区：[1]广东深圳市福田区人民医院,深圳518033 [2]嘉应学院医学院,梅州514015 [3]深圳市药品检验所,深圳518057

出　　处：《海峡药学》2015年第1期43-44,共2页Strait Pharmaceutical Journal

摘　　要：目的建立复方苦参败酱草保留灌肠剂中齐墩果酸的含量测定方法。方法采用高效液相色谱法,色谱柱为phenomenex lune（4.6×250mm,5μm）,流动相为乙睛-水（90∶10）,流速为1.0m L·min^-1,检测波长为210nm,柱温25℃。结果齐墩果酸的检测线性范围为20~400μg·m L^-1,r=0.9998,RSD为0.46%（n=5）,平均回收率为100.34%。结论本法简便、准确、灵敏度高、重复性好,可作为复方苦参败酱草保留灌肠剂中齐墩果酸的含量测定方法。OBJECTIVE To establish a method for the determination of Oleanic Acid in compound sophora Herba Patrinia Retention enema. METHODS HPLC was used with phenomenex lune（ 4. 6 × 250 mm,5μm） column,the mobile phase was acetonitrile-water（ 90∶ 10）. The flow rate was 1. 0m L·min^- 1. The detection wavelength was 210 nm and column temperature was 25℃. RESULTS The range of inear detection of Oleanic acid was 20 ~400 gm L^-1（ r = 0. 9995）. The average recovery was 99. 6%,RSD 0. 43%（ n = 6）. CONCLUSION The method is simple,accurate,highly sensitive and well represented. It can be used for routine quality control of compound Sophora Herba Patriniae Retention enema.

关 键 词：复方苦参败酱草保留灌肠剂 齐墩果酸 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]