高效液相色谱法测定烟筋中烟碱含量  

Determination of Nicotine in Tobacco Vein by High Performance Liquid Chromatography

作　　者：高保卫[1] 董艳玲[1] 张璟[1] 冯俊涛[1] 王智辉[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心/陕西省生物农药工程技术研究中心,陕西杨凌712100

出　　处：《农药》2015年第3期194-196,共3页Agrochemicals

基　　金：国家自然科学基金项目(31272110);西北农林科技大学大型仪器新功能开发项目(dysb1321)

摘　　要：[目的]建立高效液相色谱测定烟筋中的烟碱含量。[方法]用4%Na OH溶液提取,正己烷和20%H2SO4溶液萃取,Ba（OH）2沉淀的方法提取烟筋中烟碱;选用Hypersil BDS-C18色谱柱,流动相为甲醇-水（0.02 mol磷酸二氢钾加0.52%三乙胺,用磷酸调整p H值为6.5）（体积比60颐40）,检测波长260 nm,流速1.0 m L/min,柱温40℃,进样量10滋L。[结果]烟碱质量浓度在100∽1 000 mg/L时,线性关系良好;添加水平为8.00∽32.00 mg时,回收率为98.37%--99.03%,RSD为0.65%。[结论]该方法能准确快速检测烟筋中烟碱的含量。[Aims] A HPLC method for determination of nicotine in tobacco vein was established. [Methods] The nicotine in tobacco vein was extracted with Na OH solution 4%, normal hexane and H2SO4 solution 20%, and the H2SO4 in extracts was precipitated by Ba（OH）2crystal. The nicotine in tobacco vein was determinated on Hypersil BDS-C18 chromatographic column at 40 ℃ by using methanol/water（with 0.02 mol of potassium dihydrogen phosphate and triethylamine 0.52%, and phosphoric acid was used for adjusting p H to 6.5）（60：40, by vol） as mobile phase with the flow of 1.0 m L/min, in addition, UV detection was conducted at 260 nm for 10 滋L of test sample. [Results] The results showed that the calibration curve was in good linear with nicotine content in the range of 100-1 000 mg/L. When the addition levels were 8.00-32.00 mg, the recovery rates were 98.37-99.03% with the RSD of 0.65%. [Conclusions] This method is simple with high precision and accuracy, which can be used for detection of nicotine in tobacco vein.

关 键 词：烟筋 烟碱 高效液相色谱 含量 测定 

分 类 号：O657.72[理学—分析化学] TS47[理学—化学]