盐酸金霉素分子印迹电化学传感器的研制  

Preparation of an Electrochemical Sensor for Determination of Chlortetracycline Based on Molecularly Imprinted Film

作　　者：高杨 王伟[1] 刘英姿 陶强 万雪 张娟琨[1] 

机构地区：[1]天津市工业微生物重点实验室教育部工业微生物重点实验室天津科技大学生物工程学院,天津300457

出　　处：《分析化学》2015年第2期212-217,共6页Chinese Journal of Analytical Chemistry

基　　金：天津市科委资助项目(No.14RCCFSF00140)

摘　　要：构建了一种选择性检测盐酸金霉素（ CTC）的分子印迹电化学传感器。在NaClO4溶液中，以邻氨基酚（ OAP）为功能单体，盐酸金霉素（ CTC）为模板，通过循环伏安法在玻碳电极表面上聚合制备了CTC印迹敏感膜（MIPs）。在含0.005 mol/L K3[Fe（CN）6]及0.1 mol/L KCl的磷酸盐缓冲液（PBS）中，应用差分脉冲伏安法（DPV）研究了传感器的响应性能。 DPV峰电流差与CTC浓度在2.0×10^-8~6.1×10^-7mol/L范围内呈线性关系，检出限为1.5×10^-8 mol/L （3σ）。实验表明，用甲醇/H2SO4混合洗脱溶液可以使传感器再生，对CTC的测定具有良好的重现性，并具有良好的储存稳定性。传感器对于干扰物氯霉素及青霉素没有响应，结构相似的四环素、土霉素有微弱的响应，显示了良好的选择性。在牛奶和鸡肉实际样品中所测得的CTC加标回收率为86.4%~96.9%。与文献报道的CTC检测方法相比，本传感器具有低的检测限，操作简便，整个过程无需衍生化处理，响应快，成本低。An electrochemical sensor has been developed for the selective determination of chlortetracycline （ CTC） using the molecularly imprinted technique. A molecular imprinted polymer （ MIP） on the surface of a glassy carbon electrode （ GCE ） was prepared by electropolymerization of o-aminophenol （ OAP ） in the presence of CTC in the sodium perchlorate （ NaClO4 ） solution using cyclic voltammetry （ CV ） . The electrochemical performance of the sensor was studied by using differential pulse voltammetry （ DPV ） . A linear relationship between the peak current difference and the CTC concentration was found in the range of 2. 0×10^-8-6. 1×10^-7 mol/L with the detection limit of 1. 5×10^-8 mol/L （3σ）. After regeneration by washing with the mixture of methanol and sulfuric acid, the sensor showed excellent reproducibility and good stability. The MIP electrode exhibited almost no response to chloramphenicol and penicillin, and very weak responses to tetracycline and oxytetracycline, proving a good selectivity. Recoveries of standard addition measured in the actual samples of milk and chicken meat were between 86 . 4% -96 . 9%. Compared with the reported methods, this sensor showed a low detection limit, simple operation without derivatization, rapid response and low cost.

关 键 词：分子印迹聚合物 电化学传感器 邻氨基酚 盐酸金霉素 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]