GLT-1激动剂头孢曲松钠对慢性神经病理性痛的影响  

Effect of ceftriaxone on chronic neuropathic pain and the expression of GLT-1 in rats

作　　者：卢丽莉[1] 杨倩[2] 仇艳玲 易力[1] 

机构地区：[1]河北省石家庄市第三医院病理科,050011 [2]河北省石家庄市第四医院病理科 [3]河北省优抚医院老年病科

出　　处：《河北医药》2015年第4期494-496,共3页Hebei Medical Journal

基　　金：石家庄市科学技术研究与发展指导计划项目(编号:131461223)

摘　　要：目的探讨头孢曲松钠(Ceftriaxone,Cef)对慢性神经病理性痛过敏及GLT-1表达的影响。方法雄性SD大鼠90只,随机分为Sham组、CCI 14 d组、Cef预防组、Cef治疗组,后2组设腹腔注射NS组为对照,在不同时间点测定热缩足反射潜伏期;另设CCI 1 d、4 d、7 d组。应用免疫组化观察不同时间点脊髓后角GLT-1表达的变化。结果 CCI诱导大鼠产生了热痛敏,腹腔注射NS对CCI诱导的热痛敏无影响。Cef预防组大鼠CCI侧后肢热缩足反射潜伏期于CCI后第5天、第7天明显延长(P<0.05);Cef治疗组大鼠CCI侧后肢热缩足反射潜伏期在术后第11天、第14天明显延长(P<0.05)。免疫组化检测发现,Cef预防组和Cef治疗组分别抑制和逆转了GLT-1在CCI后期表达的降低。结论 Cef可通过上调GLT-1的表达对慢性神经病理性痛起到预防和治疗作用。Objective To investigate the effects of （ Ceftriaxone ,Cef） on chronic neuropathic pain and the expression of GLT-1 in rats.Methods Ninety male Spague-Dawley rats were randomly divided into sham-operation group, CCI 14d group,Cef prevention group,Cef therapy group,at the same time,the two groups with intraperitoneal injection with NS were designed as control groups for the last two groups .The thermal withdrawal latency was measured in different time points , in addition,CCI 1d,CCI 4d and CCI 7d groups were designed to observe dynamic change of GLT-1 expression after CCI.The changes of GLT-1 expression in dorsal horn of spinal cord were detected by immunohistochemistry .Results The thermal hyperalgesia in rats was induced by chronic crushing injury （CCI）,however,intraperitoneal injection with NS had no effect on thermal hyperalgesia induced by CCI .The thermal withdrawal latency was obviously prolonged in Cef prevention group on the 5th day,the 7th day after CCI（ P 〈0.05）,moreover,which in Cef therapy group was significantly prolonged on the 11th day, 14th day after CCI（ P 〈0.05）.The results by immunohistochemisty showed that Cef prevention group and Cef therapy group inhibited and reversed the down-regulation of GLT-1 expression at CCI later period .Conclusion The Cef can prevent and treat chronic neuropathic pain by up-regulating the expression of GLT-1.

关 键 词：慢性神经病理性疼痛 头孢曲松钠 GLT-1 CCI 

分 类 号：R441.1[医药卫生—诊断学]